The Effect of Linseed on Intramuscular Fat Content and Adipogenesis Related Genes in Skeletal Muscle of Pigs
The Effect of Linseed on Intramuscular Fat Content and Adipogenesis Related Genes in Skeletal Muscle of Pigs
复制标题
亚麻籽对猪骨骼肌肌内脂肪含量及脂肪生成相关基因的影响
DOI：
10.1007/s11745-009-3346-y
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发表时间：
2009-11-01
期刊：
影响因子：
1.9
通讯作者：
Peng, Jian
中科院分区：
文献类型：
--
作者：
Luo, He-Feng;Wei, Hong-Kui;Peng, Jian
本研究的目的是研究最长肌中n - 3 PUFA的富集对生长-肥育母猪肌内脂肪（IMF）含量和相关基因表达的影响。配制两种等能、等氮、等脂饲料：一种为基础饲料，另一种为添加10%亚麻籽的饲料。选取24头体重为35±3.7 kg的长白×新丹兰母猪，随机分为4个处理组，每组6头猪。试验期90 d，各组分别在屠宰前0、30、60、90 d先饲喂基础饲粮，再饲喂亚麻籽饲粮。测量和分析了最长肌的肉质、脂肪酸组成和脂肪形成相关基因的表达。随着饲粮饲喂时间的延长，饲料中IMF含量呈线性增加（P< 0.05）。同时，n‐3 PUFA含量和过氧化物酶体增殖物激活受体δ(PPARδ)、PPARγ、脂肪细胞脂肪酸结合蛋白（aP2）、脂蛋白脂肪酶（LPL）的表达呈线性增加（P< 0.01），无翼相关MMTV整合位点10b（Wnt10b）的表达呈线性下降（P< 0.01）。此外，n‐3 PUFA浓度与这些基因的表达呈极显著（P< 0.01）二次或线性关系，ppar γ、ap2或wnt10波段IMF含量与表达呈极显著（P< 0.01）二次或线性关系。这些数据表明，增强肌肉中n - 3 PUFA的富集会导致IMF含量显著增加。一种可能的解释是由于参与脂肪形成的基因表达的改变，然而这需要通过蛋白质和酶活性研究来证实。
The aim of the study was to investigate the effect of n‐3 PUFA enrichment in longissimus muscle on intramuscular fat (IMF) content and expression of related genes in growing‐finishing barrows. Two isoenergetic, isonitrogenous and isolipidic diets were formulated: one was basal diet and the other contained 10% linseed. Twenty‐four Landrace × NewDamLine barrows weighing 35 ± 3.7 kg were randomly assigned to four treatment groups with six pigs per group. During the whole experimental period of 90 days, all groups were first fed the basal diet and then the linseed diet for 0, 30, 60, and 90 days before slaughter, respectively. Meat quality, fatty acid composition, and expression of genes involved in adipogenesis in longissimus muscle were measured and analyzed. The IMF content increased linearly (P< 0.05) as the linseed diet feeding time prolonged. Meanwhile, n‐3 PUFA content and expression ofperoxisome proliferator‐activated receptor δ(PPARδ),PPARγ,adipocyte fatty acid–binding protein(aP2) andlipoprotein lipase(LPL) increased linearly (P< 0.01) as well, while the expression ofwingless related MMTV integration site 10b(Wnt10b) linearly decreased (P< 0.01). Furthermore, significant (P< 0.01) quadratic or linear relation was observed between n‐3 PUFA enrichment and expression of these genes, while significant (P< 0.01) quadratic or linear relation was observed between the expression ofPPARγ,aP2orWnt10band IMF content. These data show that enhancing n‐3 PUFA enrichment in muscle leads to significant increase in IMF content. A possible explanation is due to alterations in the expression of genes involved in adipogenesis, however this will need to be confirmed by protein and enzyme activity studies.