Functional region mapping of HIV-2 Vpx protein
Functional region mapping of HIV-2 Vpx protein
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DOI：
10.1016/j.micinf.2008.08.005
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发表时间：
2008-10-01
影响因子：
5.8
通讯作者：
Adachi, Akio
中科院分区：
文献类型：
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作者：
Fujita, Mikako;Otsuka, Masami;Adachi, Akio
为了确定HIV-2 Vpx的功能区域，我们分析了一系列位点特异性Vpx突变体在淋巴细胞中的生长潜力，并将结果与巨噬细胞中的结果进行了比较。我们发现在淋巴细胞、巨噬细胞和两者中对病毒生长重要的氨基酸残基分别聚集在Vpx中。通过新的Vpx突变体的产生和表征，我们进一步证明了Vpx的c端存在显著的脯氨酸延伸，这对其稳定表达至关重要，从而有助于其功能活性。综上所述，在HIV-2 Vpx中可能存在功能不同的区域。(C) 2008 Elsevier Masson SAS。版权所有。
To determine functional regions of HIV-2 Vpx, we analyzed a series of site-specific vpx-mutants for their growth potentials in lymphocytic cells and compared the results with those in macrophages. We found that amino acid residues important for virus growth in lymphocytic cells, in macrophages, and in both are clustered separately in Vpx. Through generation and characterization of new vpx-mutants, we further demonstrated that a remarkable proline-stretch present at the C-terminus of Vpx is critical for its stable expression, thereby contributing to its functional activity. Taken together, there can be functionally distinct regions in HIV-2 Vpx. (C) 2008 Elsevier Masson SAS. All rights reserved.