Ultrastructure of absorbing peripheral lymphatic vessel (ALPA) in guinea pig Peyer's patches
Ultrastructure of absorbing peripheral lymphatic vessel (ALPA) in guinea pig Peyer's patches
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DOI：
10.1006/mvre.2002.2428
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发表时间：
2002-09-01
影响因子：
3.1
通讯作者：
Arcari, ML
中科院分区：
文献类型：
--
作者：
Azzali, G;Vitale, M;Arcari, ML
氯丁橡胶直接注入豚鼠肠道相关淋巴组织中的淋巴管和血管，即孤立淋巴滤泡和Peyer‘s斑块，在饥饿和淋巴滞留后的生理条件下，腐蚀铸型既显示了其数密度，也显示了它们的地形排列。吸收的外周淋巴管(ALPA)始于乳管，在粘膜淋巴管网络中延续。后者是由上皮下血管和滤泡间血管包裹单个淋巴滤泡形成的篮子。滤泡间血管流出粘膜下层，流入带有非节段性二尖瓣的肌层血管。它们依次排出浆膜下集液器中的淋巴，然后排出具有传导功能的淋巴结前集器。卵泡的生发中心和顶端完全没有ALPA血管，而它们却有丰富的血管。在超微结构上，ALPA血管壁由单层内皮细胞组成，无孔、开窗和开放连接，缺乏连续的基底膜。内皮细胞通过重叠和交错的细胞间接触连接在一起，而端到端的接触很少见。它们具有相当大的细胞体，包括细胞核和共同的内质细胞器，以及外围细胞质，具有肌动蛋白样丝束、游离微泡或形成通道和少数表面粗糙的包膜质网(RER)小管。在细胞从淋巴样组织向淋巴管腔的跨内皮细胞迁移的不同阶段，内皮壁中常常可以检测到淋巴细胞跨越内皮细胞通道的存在。这些通道在饥饿期间数量增加，而在淋巴滞留期间稀少。在生理和实验条件下(饥饿、淋巴滞留)，我们定量评估了滤泡间ALPA血管淋巴液中T淋巴细胞的发生率，以及有或没有Peyer‘s补片引流小肠的淋巴结前收集器的发生率。(C)2002年埃尔塞维尔科学公司(美国)。
Corrosion casts from neoprene direct injection of lymphatic and blood vessels in guinea pig gut-associated lymphoid tissue, that is, solitary lymphoid follicles and Peyer's patches, have shown both their numerical density and their topographical arrangement in physiological conditions, after starvation and lymphatic stasis. The absorbing peripheral lymphatic vessel (ALPA) begins with the lacteal vessel, which continues in the mucosal lymphatic network. The latter is formed by subepithelial and interfollicular vessels wrapping single lymphoid follicles like a basket. Interfollicular vessels drain in the submucosal network, which flows into muscular tunica vessels with nonsegmentary bicuspid valves. They in turn drain lymph in subserosal precollectors and then in prelymphonodal collectors with conduction function. The follicles' germinal center and dome are completely devoid of ALPA vessels, while they are rich in blood vessels. Ultrastructurally, the ALPA vessel wall consists of a monolayer of endothelial cells devoid of pores, fenestrations, and open junctions and lacking a continuous basal lamina. Endothelial cells are joined by overlapping and interdigitating intercellular contacts, while end-to-end contacts are rare. They have a sizeable cell body, containing the nucleus and the common endocytoplasmic organelles, and a peripheral cytoplasm with actin-like filament bundles, free microvesicles or forming channels and a few rough-surfaced encloplasmic reticulum (RER) canaliculi. The presence of intraendothelial channels crossed by lymphocytes can often be detected within the endothelial wall during the different phases of cell transendothelial migration from lymphoid tissue to lymphatic vessel lumen. These channels undergo a numerical increase during starvation, while they are scarce during lymphatic stasis. We have quantitatively evaluated the prevalence of T lymphocytes in the lymph of interfollicular ALPA vessels and of prelymphonodal collectors draining the small intestine tract with or without Peyer's patches, under physiological and experimental conditions (starvation, lymphatic stasis). (C) 2002 Elsevier Science (USA).