LONG-TERM HUMAN CYTOLYTIC T-CELL LINES ALLOSPECIFIC FOR HLA-DR6 ANTIGEN ARE OKT4+
LONG-TERM HUMAN CYTOLYTIC T-CELL LINES ALLOSPECIFIC FOR HLA-DR6 ANTIGEN ARE OKT4+
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DOI：
10.1073/pnas.79.7.2365
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
BURAKOFF, SJ
中科院分区：
文献类型：
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作者：
KRENSKY, AM;REISS, CS;BURAKOFF, SJ
人同种异体细胞毒性T淋巴细胞（CTL）系的建立，每周刺激外周血淋巴细胞与异基因EB病毒转化的淋巴母细胞系的存在下，白细胞介素2。细胞毒性T淋巴细胞系由JY（其表达HLA-A、B和-DR）或Daudi（其表达HLA-DR但不表达-A、B抗原）刺激。通过抗体阻断和针对一组靶细胞系的细胞溶解模式确定效应细胞系的特异性。通过阴性（抗体和补体裂解）和阳性（荧光激活细胞分选仪）选择鉴定效应细胞的表型。抗JY系仅识别携带HLA-A2或B7抗原的靶细胞，并且仅由OKT 4 - 8+效应细胞组成。抗Daudi细胞系特异性识别携带DR6的靶标，最终仅含有OKT 4 + 8-细胞。因此，Daudi细胞的刺激可导致产生对DR6抗原具有同种特异性的OKT 4 + CTL系。
Human allospecific cytotoxic T lymphocyte (CTL) lines were established by weekly stimulation of peripheral blood lymphocytes with allogeneic Epstein-Barr virus-transformed lymphoblastoid cell lines in the presence of interleukin 2. The cytotoxic T lymphocyte lines were stimulated by either JY (which expresses HLA-A,B and -DR) or Daudi (which expresses HLA-DR but not -A,B antigens). Specificity of the effector cell lines was determined by antibody blocking and by patterns of cytolysis against a panel of target cell lines. The phenotype of the effector cells was identified by both negative (antibody and complement lysis) and positive (fluorescence-activated cell sorter) selection. The anti-JY lines only recognized targets bearing HLA-A2 or B7 antigens and were composed exclusively of OKT4-8+ effector cells. The anti-Daudi lines specifically recognized targets bearing DR6 and eventually contained only OKT4+8- cells. Thus, stimulation by Daudi cells can result in the generation of OKT4+ CTL lines that are allospecific for DR6 antigen.