Development of reverse transcription loop mediated isothermal amplification assay for rapid detection of bluetongue viruses
Development of reverse transcription loop mediated isothermal amplification assay for rapid detection of bluetongue viruses
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DOI：
10.1016/j.jviromet.2015.06.005
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发表时间：
2015-09-15
影响因子：
3.1
通讯作者：
Mondal, Bimalendu
中科院分区：
文献类型：
--
作者：
Mohandas, Sreekala S.;Muthuchelvan, Dhanavelu;Mondal, Bimalendu
以BTV-1、BTV-2、BTV-9、BTV-10、BTV-16、BTV-21和BTV-23 7种血清型为对象，对单步逆转录环介导等温扩增（RT-LAMP）方法进行了优化和验证。该方法的相对灵敏度为0.3 TCID50，与口蹄疫、小反刍兽疫、羊痘、羊痘和口蹄疫病毒无交叉反应性。建立的检测方法还通过筛选临床样本进行了评估，结果与传统的RT-PCR相当。本文所述的RT-LAMP测定法可作为现有BTV诊断/监测测定法的补充工具。(C) 2015 Elsevier B.V.版权所有
A single-step reverse transcription loop mediated isothermal amplification (RT-LAMP) assay targeting NS1 - a highly conserved gene among BTV serotypes was optimized and validated with seven serotypes: BTV-1, BTV-2, BTV-9, BTV-10, BTV-16, BTV-21 and BTV-23. The relative sensitivity of the assay was 0.3 TCID50 and no cross reactivity could be observed with foot and mouth disease, peste-des-petits-ruminants, goatpox, sheeppox and orf viruses. The established assay was also assessed by screening of clinical samples and the result is comparable with conventional RT-PCR. The RT-LAMP assay described here could be an additional tool to the existing assays for diagnosis/surveillance of BTV. (C) 2015 Elsevier B.V. All rights reserved.