The Wax–Liquid Transition Modulates Hydrocarbon Respiration Rates in Alcanivorax borkumensis SK2
The Wax–Liquid Transition Modulates Hydrocarbon Respiration Rates in Alcanivorax borkumensis SK2
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蜡-液体转变调节 Alcanivorax borkumensis SK2 的碳氢化合物呼吸速率
DOI：
10.1021/acs.estlett.8b00143
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发表时间：
2018
影响因子：
10.9
通讯作者：
Valentine, David L.
中科院分区：
文献类型：
--
作者：
Lyu, Li-Na;Ding, Haibing;Cui, Zhisong;Valentine, David L.
海洋碳氢化合物的生物降解是一个重要的环境过程，由微生物进行，并受海洋条件的调节。根据深水地平线灾难后观察到的石油烃生物降解模式，我们测量了专性烷烃降解细菌Alcanivorax borkumensisSK2的呼吸速率，以评估碳氢化合物呼吸速率与基质相（蜡质与液体）之间的关系。使用温度（20、25、30、35和40°C）和正构烷烃（n-C14、n-C15、n-C16、n-C17、n-C18、n-C19和n- c20）的矩阵，其中每个温度间隙跨越了正构烷烃的相变点，我们证明，当底物处于蜡相时，相对于液相，正构烷烃呼吸速率显著降低。观察到的效果跨越了整个实验温度范围。随后仅用蜡相c19进行的实验表明，表面积的可用性调节了烷烃的呼吸速率，并且可能是导致蜡相的呼吸速率低于液相碳氢化合物的呼吸速率的一个因素。这些结果表明，蜡相烃易被a降解。borkumensisSK2，但相对于液相碳氢化合物的速率被抑制了。该结果与深水地平线对碳氢化合物生物降解模式的解释一致，与海洋中碳氢化合物的行为具有更广泛的相关性。
Marine hydrocarbon biodegradation is an important environmental process conducted by microbes and modulated by oceanographic conditions. Following up on the patterns of petroleum hydrocarbon biodegradation observed after theDeepwater Horizondisaster, we measured respiration rates for the obligate alkane-degrading bacterium,Alcanivorax borkumensisSK2, to assess the relationship between hydrocarbon respiration rates and the phase, wax versus liquid, of the substrate. Using a matrix of temperatures (20, 25, 30, 35, and 40 °C) andn-alkanes (n-C14,n-C15,n-C16,n-C17,n-C18,n-C19, andn-C20) for which each temperature gap spans the phase transition point of ann-alkane, we demonstrate that then-alkane respiration rate decreases substantially when the substrate is in the wax versus liquid phase. The observed effect spans the full experimental temperature range. Subsequent experimentation with only wax-phasen-C19indicates that the availability of surface area modulates then-alkane respiration rate and is likely a factor contributing to the observed respiration rates being lower for wax-phase than for liquid-phase hydrocarbons. These results demonstrate that wax-phase hydrocarbons are subject to biodegradation byA. borkumensisSK2 but that rates are suppressed relative to those of liquid-phase hydrocarbons. The results are consistent with interpretations of hydrocarbon biodegradation patterns fromDeepwater Horizonwith broader relevance to the behavior of hydrocarbons in the ocean.