Evaluation of albumin concentration in rabbit anterior chamber with laser flare-cell meter.
Evaluation of albumin concentration in rabbit anterior chamber with laser flare-cell meter.
复制标题
用激光耀斑细胞仪评价兔前房白蛋白浓度。
DOI：
10.3109/02713689508998407
复制
发表时间：
1995
影响因子：
2
通讯作者：
M. Araie
中科院分区：
文献类型：
--
作者：
M. Mori;K. Inamochi;M. Araie
为了找到一种合适的方法，使用Kowa的激光闪光细胞计在评估前房白蛋白浓度，我们的特点与闪光细胞计的闪光强度的光子计数/ms（闪光值），闪光值转换为白蛋白浓度，和前房白蛋白浓度之间的关系在药物治疗的兔眼。在接受静脉注射异硫氰酸荧光素标记的白蛋白和滴注前列腺素E1、毛果芸香碱或托吡卡胺的家兔中进行耀斑测量。测定闪光值、前房荧光、血浆荧光和血浆白蛋白浓度。根据血浆白蛋白浓度和校正的水/血浆荧光比计算前房白蛋白浓度。耀斑值转换为白蛋白浓度之间的相关性（Albequiv）与前房白蛋白浓度（Albac）之间存在两个时相：1）闪光值小于100光子数/ms时，两者呈线性相关：Albac = 0.53 Albequiv - 0.006，r = 0.98; 2）耀斑值大于100光子数/ms时，相关性较低，且斜率不同。在这种情况下，Albac与耀斑值本身的相关性更好：Albac = 0.0042 [耀斑值]+0.17，r = 0.88。结果表明，当闪光值低于100光子计数/ms时，根据转换的闪光值定量评价前房白蛋白浓度，当闪光值高于该水平时，根据闪光值本身定量评价前房白蛋白浓度。
To find an appropriate way of using Kowa's laser flare-cell meter in evaluating the anterior chamber albumin concentration, we characterized the relationship among the flare intensity determined with the flare-cell meter in terms of photon count/ms (flare value), flare value converted to albumin concentration, and anterior chamber albumin concentration in drug-treated rabbit eyes. Flare measurement was done in rabbits that received intravenous administration of fluorescein isothiocyanate-labeled albumin and instillation of prostaglandin E1, pilocarpine, or tropicamide. Flare value, anterior chamber fluorescence, plasma fluorescence, and plasma albumin concentration were determined. Anterior chamber albumin concentration was calculated from the plasma albumin concentration and the corrected aqueous/plasma fluorescence ratio. The correlation between the flare value converted to albumin concentration (Albequiv) and the anterior chamber albumin concentration (Albac) had two phases: 1) Linear correlation was found when the flare value was below 100 photon count/ms: Albac = 0.53 Albequiv - 0.006, r = 0.98; 2) When the flare value was above 100 photon count/ms, the correlation was lower and had a different slope. In this condition, the Albac was correlated better with the flare value itself: Albac = 0.0042 [flare value] + 0.17, r = 0.88. The results suggested that anterior chamber albumin concentration is evaluated quantitatively from the converted flare value when the flare value is below 100 photon count/ms and from the flare value itself when the flare value is above that level.
背景蛋白质对荧光光度数据的影响。
影响因子：
4.4
作者：
Barsotti,MF;Bartels,SP;Kamm,RD;Freddo,TF
通讯作者：
Freddo,TF
影响因子：
4.4
作者：
McLaren,JW;Trocme,SD;Relf,S;Brubaker,RF
通讯作者：
Brubaker,RF