SEROEPIDEMIOLOGICAL STUDIES OF HUMAN LYMPHOTROPIC-T RETROVIRUS TYPE-III IN ACQUIRED IMMUNODEFICIENCY SYNDROME
SEROEPIDEMIOLOGICAL STUDIES OF HUMAN LYMPHOTROPIC-T RETROVIRUS TYPE-III IN ACQUIRED IMMUNODEFICIENCY SYNDROME
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DOI：
10.1016/s0140-6736(84)91933-0
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发表时间：
1984-01-01
期刊：
影响因子：
168.9
通讯作者：
GALLO, RC
中科院分区：
文献类型：
--
作者：
SAFAI, B;SARNGADHARAN, MG;GALLO, RC
在一项双盲研究中，对34名获得性免疫缺陷综合征（AIDS）患者、19名淋巴结病综合征患者和14名患AIDS风险增加的同性恋男性的血清进行了筛选，以检测新的人类嗜T淋巴细胞逆转录病毒（白血病病毒）HTLV-III蛋白的抗体，HTLV-III是最近从AIDS患者的培养T细胞中分离出来的。在一个方便和快速的酶联免疫吸附试验和更敏感的电印迹（Western）试验的组合，100%的艾滋病血清评分阳性。在淋巴结病患者中，发现84%具有HTLV-III的血清抗体。一个较低的，但显着的比例（21%），健康的同性恋男子与艾滋病的风险增加也是积极的。没有异性恋对照，包括那些在传染性单核细胞增多症和T细胞或B细胞淋巴瘤患者的过程中与嗜异性抗体，有抗体HTLV-III。显然，HTLV-III的抗体是AIDS的诊断或指示该疾病的显著风险，并且表明HTLV-III是人类AIDS的主要原因。
In a double-blind study, sera of 34 patients with acquired immunodeficiency syndrome (AIDS), 19 patients with lymphadenopathy syndrome and 14 homosexual men with an increased risk of AIDS were screened for antibodies to proteins of the novel human T-lymphotropic retrovirus (leukemia virus), HTLV-III, recently isolated from cultured T cells of AIDS patients. On a combination of a convenient and rapid enzyme-linked immunosorbent assay and a more sensitive electroblot (Western) assay, 100% of the AIDS sera were scored positive. Of the lymphadenopathy patients, 84% were found to have serum antibodies to HTLV-III. A lower, but significant, proportion (21%) of healthy homosexual men with an increased risk of AIDS were also positive. No heterosexual controls, including those with heterophile antibodies during the course of infectious mononucleosis and patients with T-cell or B-cell lymphoma, had antibodies to HTLV-III. Evidently the antibodies to HTLV-III are diagnostic of AIDS or indicate significant risk of the disease, and suggest that HTLV-III is the primary cause of human AIDS.