Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin
Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin
复制标题
DOI：
10.1099/jmm.0.032037-0
复制
发表时间：
2011-09-01
影响因子：
3
通讯作者：
Bujdakova, Helena
中科院分区：
文献类型：
--
作者：
Kucharikova, Sona;Tournu, Helene;Bujdakova, Helena
念珠菌生物膜的发展可以受到多种因素，如底物，培养基，碳水化合物来源和pH值的影响。我们分析了生物膜形成的白色念珠菌SC5314和光滑念珠菌ATCC 2001野生型菌株在不同的介质（RPMI 1640与YNB）的存在下，并使用不同的pH值（pH 5.6或7.0）。我们确定的粘附和生物膜形成聚苯乙烯，在这些过程中的粘附素基因的表达变化和成熟的生物膜棘白菌素的敏感性。事实证明，两种念珠菌在聚苯乙烯上形成的生物膜受到培养基组成而不是pH值的强烈影响。白色念珠菌和C. glabrata在RPMI 1640培养基中形成较厚的生物膜，而在YNB培养基中，两个物种表现出粘附而不是特征性的多层生物膜结构。在pH 7.0的RPMI 1640培养基中刺激的生物膜形成与体外生物膜形成所必需的粘附素基因（包括C.白色念珠菌和EPA6。光滑的在RPMI 1640培养基中生长的较厚的生物膜比YNB生长的生物膜对卡泊芬净和阿尼芬净更耐受。我们还观察到成熟的C.在RPMI 1640培养基中，光滑藻生物膜对棘白菌素的敏感性低于C.白色念珠菌生物膜环境条件，即介质和pH值，不仅可以显着影响生物膜结构，而且还影响生物膜发育的不同阶段期间涉及的几个基因的表达谱。此外，生长条件也可能影响生物膜结构内真菌种群的抗真菌敏感性。因此，在设计任何实验生物膜设置之前，重要的是要考虑外部环境因素对念珠菌生物膜发展的潜在影响。
Candida biofilm development can be influenced by diverse factors such as substrate, culture medium, carbohydrate source and pH. We have analysed biofilm formation of Candida albicans SC5314 and Candida glabrata ATCC 2001 wild-type strains in the presence of different media (RPMI 1640 versus YNB) and using different pH values (pH 5.6 or 7.0). We determined adhesion and biofilm formation on polystyrene, changes in the expression of adhesin genes during these processes and the susceptibility of mature biofilms to echinocandins. Biofilms formed on polystyrene by both Candida species proved to be influenced strongly by the composition of the medium rather than pH. C. albicans and C. glabrata formed thicker biofilms in RPMI 1640 medium, whereas in YNB medium, both species manifested adhesion rather than characteristic multilayer biofilm architecture. The stimulated biofilm formation in RPMI 1640 medium at pH 7.0 corroborated positively with increased expression of adhesin genes, essential to biofilm formation in vitro, including ALS3 and EAP1 in C. albicans and EPA6 in C. glabrata. The thicker biofilms grown in RPMI 1640 medium were more tolerant to caspofungin and anidulafungin than YNB-grown biofilms. We also observed that mature C. glabrata biofilms were less susceptible in RPMI 1640 medium to echinocandins than C. albicans biofilms. Environmental conditions, i.e. medium and pH, can significantly affect not only biofilm architecture, but also the expression profile of several genes involved during the different stages of biofilm development. In addition, growth conditions may also influence the antifungal-susceptibility profile of fungal populations within biofilm structures. Therefore, before designing any experimental biofilm set-up, it is important to consider the potential influence of external environmental factors on Candida biofilm development.