Fate of transplanted bone-marrow-derived mesenchymal cells during osteochondral repair using transgenic rats to simulate autologous transplantation
Fate of transplanted bone-marrow-derived mesenchymal cells during osteochondral repair using transgenic rats to simulate autologous transplantation
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DOI：
10.1016/j.joca.2004.06.014
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发表时间：
2004-10-01
影响因子：
7
通讯作者：
Kubo, T
中科院分区：
文献类型：
--
作者：
Oshima, Y;Watanabe, N;Kubo, T
目的：应关注用于组织工程策略的移植细胞的命运。鉴于这一目的，移植的骨髓间充质细胞内的骨软骨缺损的生存测定使用转基因大鼠模拟autologous transplantation.Design：一个自体移植模型模拟使用转基因大鼠-其转基因不产生外源蛋白-作为供体，和野生型大鼠作为受体。采用悬滴培养技术从转基因大鼠中制备致密的间充质细胞。然后将这些细胞块移植到在野生型大鼠的内侧股骨髁内产生的骨软骨缺损中，其中它们用纤维蛋白胶固定。修复过程进行了组织学评估。结果：移植后24周，缺损处可见透明样软骨和软骨下骨形成，透明样软骨较正常软骨厚，软骨下骨形成。利用原位杂交技术，来自移植的细胞检测软骨和软骨下bone层。结论：使用这种模拟自体移植模型，移植的间充质细胞的存活在体内监测。结果表明，移植的间充质细胞有助于骨软骨缺损的修复。(C)2004年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: The fate of transplanted cells used in tissue engineering strategies should be followed. With this aim in view, the survival of transplanted bone-marrow-derived mesenchymal cells within osteochondral defects was determined using transgenic rats to simulate autologous transplantation.Design: An autologous transplantation model was simulated using transgenic rats - whose transgenes produce no foreign proteins - as donors, and wild-type rats as recipients. Dense masses of mesenchymal cells were prepared from the transgenic rats using the hanging-drop culture technique. These cell masses were then transplanted into osteochondral defects created within the medial femoral condyle of wild-type rats, wherein they are affixed with fibrin glue. The course of repair was assessed histologically. The survival of the transplanted cells was ascertained by in situ hybridization of the transgenes.Results: Twenty-four weeks after transplantation, the defects were repaired with hyaline-like cartilage, which was thicker than normal, and with subchondral bone. Using the in situ hybridization technique, cells derived from the transplanted ones were detected within both the cartilaginous and the subchondral bone layers.Conclusion: Using this simulated autologous transplantation model, the survival of transplanted mesenchymal cells was monitored in vivo. The findings indicate that the transplanted mesenchymal cells contributed to the repair of the osteochondral defects. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.