THE STRUCTURE OF SISTER MINICHROMOSOME DNA BEFORE ANAPHASE IN SACCHAROMYCES-CEREVISIAE
THE STRUCTURE OF SISTER MINICHROMOSOME DNA BEFORE ANAPHASE IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1126/science.3317838
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发表时间：
1987-12-18
期刊：
影响因子：
56.9
通讯作者：
HARTWELL, LH
中科院分区：
文献类型：
--
作者：
KOSHLAND, D;HARTWELL, LH
通过对酿酒酵母细胞周期(CDC)突变体的结构分析，研究了DNA拓扑结构在酿酒酵母细胞周期(CDC)突变体中将姐妹染色单体结合在一起的作用。在S期后后期前的大多数细胞中，姐妹微染色体分子在拓扑上互不连锁。对被释放的细胞中微小染色体倍性的分析表明，当细胞周期阻断被移除时，姐妹分子被适当地分离。因此，姊妹微染色体分子不需要保持拓扑互锁直到后期才能正确分离，而姊妹DNA分子的拓扑互锁显然不是将姐妹染色单体连接在一起的主要力量。
The role of DNA topology in holding sister chromatids together before anaphase was investigated by analyzing the structure of a small circular minichromosome in cell cycle (cdc) mutants of the yeast Saccharomyces cerevisiae. In the majority of cells arrested after S phase but before anaphase, sister minichromosome molecules are not topologically interlocked with each other. The analysis of the ploidy of minichromosomes in cells that are released from arrest demonstrates that the sister molecules are properly segregated when the cell cycle block is removed. Therefore, sister minichromosome molecules need not remain topologically interlocked until anaphase in order to be properly segregated, and topologically interlocking of sister DNA molecules apparently is not the primary force holding sister chromatids together.