QUANTIFICATION OF CELLS CULTURED ON 96-WELL PLATES
QUANTIFICATION OF CELLS CULTURED ON 96-WELL PLATES
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DOI：
10.1016/0003-2697(89)90710-0
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发表时间：
1989-10-01
影响因子：
2.9
通讯作者：
EPPENBERGER, U
中科院分区：
文献类型：
--
作者：
KUENG, W;SILBER, E;EPPENBERGER, U
本文介绍了吉利斯等（R. G.吉利斯，N. Didier，M. 03 The丹顿（1986）159，109-113）进行了修改和显著改善。由于该方法本身非常灵敏，因此该程序适用于96孔板。修饰允许染色期间细胞核吸附的染料快速且完全溶解。由于未染色或脱色细胞层的光吸收可忽略不计，因此可以在相应的威尔斯孔中进行细胞数量测量。由于这些特征，可以使用标准96孔板读数器快速进行多种测定。此外，它表明，该测定的灵敏度可以变化，很容易控制，通过选择适当的pH值在染色过程中。这增加了该方法的灵活性，使其可用于确定各种不同细胞类型的细胞密度。
The method for cell number measurement in monolayer cultures by crystal violet staining published recently by Gillies et al. (R. G. Gillies, N. Didier, M. Denton (1986) Anal. Biochem. 159, 109-113) was modified and significantly improved. The procedure was adapted for use in 96-well plates since the method is inherently very sensitive. Modifications allowed fast and complete solubilization of dye adsorbed by cell nuclei during staining. Since light absorption of the unstained or destained cell layers is negligible, cell number measurements can be performed in the respective wells. Due to these features, multiple assays may be carried out rapidly using standard 96-well plate readers. In addition, it is shown that the sensitivity of the assay can be varied and easily controlled by choosing the appropriate pH during the staining procedure. This increases the flexibility of the method making it useful for determining cell density of a wide range of different cell types.