Effect of shear stress and free radicals induced by ultrasound on erythrocytes.
Effect of shear stress and free radicals induced by ultrasound on erythrocytes.
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DOI：
10.1016/0003-9861(89)90121-5
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发表时间：
1989-03
影响因子：
3.9
通讯作者：
T. Kondo;Y. Fukushima;H. Kon;P. Riesz
中科院分区：
文献类型：
--
作者：
T. Kondo;Y. Fukushima;H. Kon;P. Riesz
本研究旨在阐明超声波诱导溶血的机制。Ar或N2O气体用于区分有或没有自由基形成（羟基自由基和氢原子）的空化。用自旋捕集结合ESR的方法检测自由基的形成。超声处理后的红细胞悬液，红细胞膜的溶血性，膜流动性，膜渗透性和膜变形性的几个结构和功能参数进行了检查。虽然自由基的形成中观察到的红细胞悬浮液中的Ar的存在下，超声处理，没有自由基的形成中观察到的N2O的存在下。然而，超声诱导的溶血行为在Ar或N2O的存在下是相似的。在Ar或N2O存在下超声处理后剩余未裂解红细胞的膜流动性、渗透性和变形性不变，与对照细胞相同。另一方面，γ射线辐照（700戈伊）后，溶血行为与超声处理后的溶血行为有很大不同，膜的性质发生了显著变化。这些结果表明，由超声处理诱导的溶血是由于机械剪切应力所产生的空化，和超声处理后剩余的红细胞的膜完整性是相同的，没有超声处理的对照细胞。三原子气体N2O可用于超声破坏细胞而不伴随自由基形成。
The present study was undertaken to elucidate the mechanism of hemolysis induced by ultrasound. Ar or N2O gas was used to distinguish between cavitation with or without free radical formation (hydroxyl radicals and hydrogen atoms). Free radical formation was examined by the method of spin trapping combined with ESR. After sonication of erythrocyte suspensions, several structural and functional parameters of the erythrocyte membrane—hemolysis, membrane fluidity, membrane permeability, and membrane deformability—were examined. Although free radical formation was observed in the erythrocyte suspensions sonicated in the presence of Ar, no free radical formation was observed in the presence of N2O. However, the hemolysis behavior induced by ultrasound was similar in the presence of Ar or N2O. The membrane fluidity, permeability, and deformability of the remaining unlysed erythrocytes after sonication in the presence of Ar or N2O were unchanged and identical to those of the control cells. On the other hand, after γ irradiation (700 Gy), the hemolysis behavior was quite different from that after sonication, and the membrane properties were significantly changed. These results suggest that hemolysis induced by sonication was due to mechanical shearing stress arising from cavitation, and that the membrane integrity of the remaining erythrocytes after sonication was the same as that of control cells without sonication. The triatomic gas, N2O, may be useful for ultrasonically disrupting cells without accompanying free radical formation.