Cutting edge: Profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues
Cutting edge: Profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues
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DOI：
10.4049/jimmunol.170.3.1136
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发表时间：
2003-02-01
影响因子：
4.4
通讯作者：
Yoshie, O
中科院分区：
文献类型：
--
作者：
Nakayama, T;Hieshima, K;Yoshie, O
我们系统地研究了人类浆细胞表达的趋化因子受体库。新鲜骨髓浆细胞和骨髓瘤细胞一致地表达CXCR 4、CXCR 6、CCR 10和CCR 3。因此，浆细胞作出反应。它们各自的配体在趋化性和非常晚的Ag-4依赖性细胞粘附到纤连蛋白中。固定化CXC趋化因子配体（CXCL）16，一种新的跨膜型趋化因子和CXCR 6配体，也直接诱导浆细胞的粘附，而不需要G（α）信号传导或二价阳离子。此外，我们揭示了CXCL 12（CXCR 4配体）、CXCL 16（CXCR 6配体）和CC趋化因子配体28（CCR 10和CCR 3配体）在富含浆细胞的组织（包括骨髓）中的一致表达和组成型表达。CXCL 12、CXCL 16和CC趋化因子配体28的表达。总的来说，浆细胞可能通过CXCR 4、CXCR 6、CCR 10和CCR 3募集到骨髓和其他靶组织。CXCR 6还可以通过其与膜锚定的CXCL 16的直接结合而有助于浆细胞的组织定位。
We systematically examined the repertoire of chemokine receptors expressed by human plasma cells. Fresh bone marrow plasma cells and myeloma cells consistently expressed CXCR4, CXCR6, CCR10, and CCR3. Accordingly, plasma cells responded to. their respective ligands in chemotaxis and very late Ag-4-dependent cell adhesion to fibronectin. Immobilized CXC chemokine ligand (CXCL)16, a novel transmembrane-type chemokine and CXCR6 ligand, also directly induced adhesion of plasma cells without requiring G(alphai) signaling or divalent cations. Furthermore, we revealed consistent expression of CXCL12 (CXCR4 ligand), CXCL16 (CXCR6 ligand), and CC chemokine ligand 28 (CCR10 and CCR3 ligand) in tissues enriched with plasma cells including bone marrow, and constitutive expression. of CXCL12, CXCL16, and CC chemokine ligand 28 by cultured human bone marrow stromal cells. Collectively, plasma cells are likely to be recruited to bone marrow and other target tissues via CXCR4, CXCR6, CCR10, and CCR3. CXCR6 may also contribute to tissue localization of plasma cells through its direct binding to membrane-anchored CXCL16.