Selection of broad-spectrum cephalosporin-resistant Escherichia coli in the feces of healthy dogs after administration of first-generation cephalosporins
Selection of broad-spectrum cephalosporin-resistant Escherichia coli in the feces of healthy dogs after administration of first-generation cephalosporins
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DOI：
10.1111/1348-0421.12466
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发表时间：
2017-01-01
影响因子：
2.6
通讯作者：
Asai, Tetsuo
中科院分区：
文献类型：
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作者：
Kimura, Ayako;Yossapol, Montira;Asai, Tetsuo
虽然抗菌产品对于治疗细菌引起的疾病至关重要，但抗菌治疗选择抗菌素耐药（AMR）细菌。本研究的目的是确定第一代头孢菌素对犬粪便中耐药大肠杆菌的影响。头孢氨苄（LEX）耐药大肠杆菌的比例明显高于头孢氨苄耐药大肠杆菌。使用含或不含LEX（50 μ g/mL）的DHL琼脂检测去势前静脉注射头孢唑啉，然后术后口服LEX 3天的3只健康狗粪便样品中的大肠杆菌。抗LEX E.大肠埃希菌在3天内检出率为100%。在所有狗中，PO 3-5天，并且主要直到PO 12天才被发现。抗LEX E.大肠杆菌菌株在含有LEX的DHL琼脂上进行抗菌药物敏感性测试、脉冲场凝胶电泳（PFGE）基因分型、β-内酰胺酶分型和质粒谱分析。所有测试的分离株均表现出头孢噻肟（CTX）抗性（CTX最小抑制浓度>= 4 μ g/mL）。将7个PFGE图谱分为5组和3种b-内酰胺酶组合（bla（CMY-4）-bla（TEM-1）、bla（TEM-1）-bla（CTX-M-15）和bla（TEM-1）-bla（CTX-M-15）-bla（CMY-4））。所有分离株在PO第4天在所有犬中均表现出相同的PFGE图谱，随后表现出不同的PFGE图谱。我们的研究结果表明，有两个选择期的AMR细菌产生的使用抗菌药物。因此，持续的卫生习惯是必要的，以防止抗菌剂给药后AMR细菌通过犬粪便转移。
Although antimicrobial products are essential for treating diseases caused by bacteria, antimicrobial treatment selects for antimicrobial-resistant (AMR) bacteria. The aim of this study was to determine the effects of administration of first-generation cephalosporins on development of resistant Escherichia coli in dog feces. The proportions of cephalexin (LEX)-resistant E. coli in fecal samples of three healthy dogs treated i.v. with cefazolin before castration and then orally with LEX for 3 days post-operation (PO) were examined using DHL agar with or without LEX (50 mu g/mL). LEX-resistant E. coli were found within 3 days PO, accounted for 100% of all identified E. coli 3-5 days PO in all dogs, and were predominantly found until 12 days PO. LEX-resistant E. coli isolates on DHL agar containing LEX were subjected to antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE) genotyping, beta-lactamase typing and plasmid profiling. All isolates tested exhibited cefotaxime (CTX) resistance (CTX minimal inhibitory concentration >= 4 mu g/mL). Seven PFGE profiles were classified into five groups and three b-lactamase combinations (bla(CMY-4)-bla(TEM-1), bla(TEM-1)-bla(CTX-M-15) and bla(TEM-1)-bla(CTX-M-15)-bla(CMY-4)). All isolates exhibited identical PFGE profiles in all dogs on four days PO and subsequently showed divergent PFGE profiles. Our results indicate there are two selection periods for AMR bacteria resulting from the use of antimicrobials. Thus, continuing hygiene practices are necessary to prevent AMR bacteria transfer via dog feces after antimicrobial administration.