Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes
Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes
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DOI：
10.1006/taap.1997.8130
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发表时间：
1997-06-01
影响因子：
3.8
通讯作者：
Gebhardt, R
中科院分区：
文献类型：
--
作者：
Gebhardt, R
用体外培养的大鼠原代肝细胞分别暴露于叔丁基过氧化氢（t-BHP）和枯烯过氧化氢苷（cumene hydroperoside），研究朝鲜蓟叶水溶性提取物的抗氧化和保护作用。这两种过氧化氢都能刺激丙二醛（hIDA）的产生，特别是当细胞用二乙基丙氨酸（DEM）预处理以降低细胞谷胱甘肽（GSH）水平时，添加朝鲜蓟提取物不影响基础MDA的产生，但当同时或在过氧化物之前存在时，以浓度依赖性方式防止过氧化氢诱导的MDA形成增加，有效浓度（低至0.001mg/ml）远低于开始高于1 mg/ml的提取物的细胞毒性水平，通过LDH渗漏试验和MTT试验评估的保护潜力与MDA产生的减少密切相关，并且在很大程度上防止了由氢过氧化物诱导的肝细胞坏死。朝鲜蓟提取物不影响细胞水平的谷胱甘肽（GSH），但减少了t-BHP暴露引起的总GSH的损失和GSSG的细胞渗漏。绿原酸和洋蓟苷仅占提取物抗氧化成分的一部分，对胰蛋白酶消化、煮沸、酸化等处理有抵抗作用，对碱化稍敏感。这些结果表明，朝鲜蓟提取物具有显着的抗氧化和保护潜力，原代肝细胞培养似乎适合于识别负责这些效果的成分，并阐明其可能的作用模式。(C)北京：科学出版社.
Primary rat hepatocyte cultures exposed to tert-butylhydroperoxide (t-BHP) or cumene hydroperoside were used to assess the antioxidative and protective potential of water-soluble extracts of artichoke leaves, Both hydroperoxides stimulated the production of malondialdehyde (h IDA), particularly when the cells were pretreated with diethyimaleate (DEM) in order to diminish the level of cellular glutathione (GSH), Addition of artichoke extracts did not affect basal MDA production, but prevented the hydroperoxide-induced increase of MDA formation in a concentration-dependent manner when presented simultaneously or prior to the peroxides, The effective concentrations (down to 0.001 mg/ml) were well below the cytotoxic levels of the extracts which started above 1 mg/ml, The protective potential assessed by the LDH leakage assay and the MTT assay closely paralleled the reduction in MDA production and largely prevented hepatocyte necrosis induced by the hydroperoxides, The artichoke extracts did not affect the cellular level of glutathione (GSH), but diminished the loss of total GSH and the cellular leakage of GSSG resulting from exposure to t-BHP. Chlorogenic acid and cynarin accounted for only part of the antioxidative principle of the extracts which was resistent against tryptic digestion, boiling, acidification, and other treatments, hut was slightly sensitive to alkalinization. These results demonstrate that artichoke extracts have a marked antioxidative and protective potential, Primary hepatocyte cultures seem suitable for identifying the constituents responsible for these effects and for elucidating their possible mode of action. (C) 1997 Academic Press.