Optimization of Growth Condition of Vibrio parahaemolyticus via Response Surface Methodology
Optimization of Growth Condition of Vibrio parahaemolyticus via Response Surface Methodology
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作者：
L. Dai
通过单因素分析和响应面分析确定了VpJ33生长的最适pH值、最适温度和最适盐度。采用多元非线性模型研究了VpJ33的最适生长条件：pH值为8.41，温度为34.1℃，盐度为2.47%。在发芽过程中，pH与盐度、pH和温度之间均存在显著的交互作用。实验结果表明，该模型具有较高的精度，可用于描述和预测VPJ33在不同外界环境因子下的生长特征。
In this paper,the optimum pH,temperature and salinity for VpJ33 growth were determined through singe-factor analysis respectively and the Response Surface Methodology(RSM).The multivariate non-linear model was applied to study the interactions and optimization of the growth parameters for VpJ33 which were explored by experiments.The experimental results showed that the optimum conditions for germination as follows:pH 8.41,temperature 34.1℃ and salinity 2.47%.Significant interactions between pH and salinity,as well as pH and temperature were found during the germination respectively,otherwise the relationship between salinity and temperature was relatively unapparent.The experimental results indicated that the proposed model is reasonably accurate and can be used for describing and predicting the growth feature of VPJ33 in different outside environmental factors.