Protocol for mapping double-stranded DNA break sites across the genome with translocation capture sequencing.
Protocol for mapping double-stranded DNA break sites across the genome with translocation capture sequencing.
复制标题
通过易位捕获测序绘制基因组中双链 DNA 断裂位点的方案。
DOI：
10.1016/j.xpro.2023.102205
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
LaSpada,AlbertR
中科院分区：
文献类型：
--
作者：
Delaney,JoeR;LaSpada,AlbertR
Translocation sequencing can be used to assess mechanisms of DNA repair and identify genome-wide double-strand breaks (DSBs) accessible to DNA repair machinery. Here, we present a protocol for mapping double-strand DNA break sites across the genome with translocation capture sequencing. Bait DSBs are introduced using a Cas9 nuclease and repaired by the host cell, connecting bait DSBs to other DSBs. Repair sites are detected by isolating bait site DNA, cleaving normal sequence to enrich off-site repair, and next-generation sequencing.For complete details on the use and execution of this protocol, please refer to Switonski et al. (2021).1
