LOCALIZATION OF SOME ENZYMES OF BETA-OXIDATION OF FATTY-ACIDS IN PEROXISOMES OF TETRAHYMENA
LOCALIZATION OF SOME ENZYMES OF BETA-OXIDATION OF FATTY-ACIDS IN PEROXISOMES OF TETRAHYMENA
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DOI：
10.1111/j.1550-7408.1973.tb03600.x
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发表时间：
1973-01-01
期刊：
影响因子：
--
通讯作者：
BLUM, JJ
中科院分区：
文献类型：
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作者：
BLUM, JJ
用蔗糖梯度沉降法从梨形四膜虫（Tetrahymena pyriformis）的匀浆中制备线粒体和过氧化物酶体。过氧化氢酶和异柠檬酸裂解酶作为过氧化物酶体标记;乳酸脱氢酶和谷氨酸脱氢酶作为线粒体标记。在过氧化物酶体和线粒体中均发现乙酰辅酶A合成酶、辛酰辅酶A合成酶、棕榈酰辅酶A合成酶、3-β羟酰辅酶A脱氢酶和硫解酶活性。结果表明，在四膜虫的两种细胞器中都存在脂肪酸的β-氧化。
Mitochondria and peroxisomes were prepared from homogenates ofTetrahymena pyriformisby sedimentation through sucrose gradients. Catalase and isocitrate lyase served as peroxisomal markers; lactic dehydrogenase and glutamic dehydrogenase as mitochondrial markers. Acetyl‐CoA synthetase, octanoyl‐CoA synthetase, palmitoyl‐CoA synthetase, 3‐βhydroxyacyl CoA dehydrogenase, and thiolase activities were found in both the peroxisomes and the mitochondria. It is suggested thatβ‐oxidation of fatty acids accurs in both organelles inTetrahymena.