Diversity of centromeric repeats in two closely related wild rice species, Oryza officinalis and Oryza rhizomatis
Diversity of centromeric repeats in two closely related wild rice species, Oryza officinalis and Oryza rhizomatis
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两种密切相关的野生稻物种（Oryza officinalis 和 Oryza rhizomatis）着丝粒重复的多样性
DOI：
10.1007/s00438-006-0103-2
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发表时间：
2006-05-01
影响因子：
3.1
通讯作者：
Cheng, Zhukuan
中科院分区：
文献类型：
--
作者：
Bao, Weidong;Zhang, Wenli;Cheng, Zhukuan
Oryza officinalis（CC，2n=24）和Oryza rhizomatis（CC，2n=24）属于稻属（Oryza）植物，其中包括20多个已鉴定的野生稻种。尽管人们对水稻着丝粒的分子组成和组织结构已经有了很多了解，但对其野生近缘种却知之甚少。在本研究中，我们分离和鉴定了一个126 bp的着丝粒卫星（CentO-C）从三个细菌人工染色体的O。药用植物除CentO-C外，O.药用植物为了确定CentO-C（126 bp）、CentO（155 bp）和TrsC（366 bp）卫星在O.在粗线期或中期I染色体上进行荧光原位杂交检测。我们发现O.着丝粒7包含CentO卫星，而着丝粒2没有任何可检测的卫星。对于TrsC卫星，在O. officinalis，O. Rhizomatis中，TrsC序列在4个着丝粒区（CEN 3、4、10、11）和多个亚端粒区均被检测到。因此，这些数据揭示了水稻近缘种内或种间着丝粒DNA的进化多样性模式，为深入了解水稻着丝粒的动态进化过程提供了依据。
Oryza officinalis (CC, 2n=24) and Oryza rhizomatis (CC, 2n=24) belong to the Oryza genus, which contains more than 20 identified wild rice species. Although much has been known about the molecular composition and organization of centromeres in Oryza sativa, relatively little is known of its wild relatives. In the present study, we isolated and characterized a 126-bp centromeric satellite (CentO-C) from three bacterial artificial chromosomes of O. officinalis. In addition to CentO-C, low abundance of CentO satellites is also present in O. officinalis. In order to determine the chromosomal locations and distributions of CentO-C (126-bp), CentO (155 bp) and TrsC (366 bp) satellite within O. officinalis, fluorescence in situ hybridization examination was done on pachytene or metaphase I chromosomes. We found that only ten centromeres (excluding centromere 7 and 2) contain CentO-C arrays in O. officinalis, while centromere 7 comprises CentO satellites, and centromere 2 is devoid of any detectable satellites. For TrsC satellites, it was detected at multiple subtelomeric regions in O. officinalis, however, in O. rhizomatis, TrsC sequences were detected both in the four centromeric regions (CEN 3, 4, 10, 11) and the multiple subtelomeric regions. Therefore, these data reveal the evolutionary diversification pattern of centromere DNA within/or between close related species, and could provide an insight into the dynamic evolutionary processes of rice centromere.