Expression of the Fas antigen in patients infected with human immunodeficiency virus.
Expression of the Fas antigen in patients infected with human immunodeficiency virus.
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DOI：
10.1002/cyto.990220206
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发表时间：
1995-06
期刊：
影响因子：
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通讯作者：
T. McCloskey;N. Oyaizu;M. Kaplan;S. Pahwa
中科院分区：
文献类型：
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作者：
T. McCloskey;N. Oyaizu;M. Kaplan;S. Pahwa
来自HIV感染患者的淋巴细胞已被证明经历了加速的凋亡。Fas抗原是一种已知的启动细胞凋亡信号的细胞表面蛋白。因此，我们开展了一项研究，检测Fas抗原在HIV感染过程中的表达。应用三色流式细胞术检测了23例HIV感染者(CDC-2型，CD4200-499细胞/微升，n=10；CDC-3型，CD4<；200细胞/微升，n=13)和10例健康对照淋巴细胞表面Fas抗原的表达。检测CD3+CD4+和CD3+CD8+亚群的表达。在健康对照组淋巴细胞中，47%的CD3+CD4+细胞和45%的CD3+CD8+细胞Fas抗原阳性。在属于CDC 3类的HIV感染患者的CD4细胞中，这一比例显著增加，但与CDC 2类受试者的正常值没有变化。患者中表达Fas抗原的CD_4~+T细胞百分比的增加与循环CD_4 T细胞计数的降低显著相关(P<；0.009)。此外，通过检测平均荧光强度，我们发现在第3类患者中，CD3+CD4+细胞的Fas表达量增加了3倍，CD3+CD8+细胞的Fas表达量增加了2倍。这些结果表明，在HIV感染过程中，Fas抗原表达增加。
Lymphocytes from patients with HIV infection have been shown to undergo accelerated apoptosis. Fas antigen is a cell surface protein known to initiate an apoptotic signal. Therefore, we undertook a study to examine the expression of the Fas antigen during HIV infection. Using three color flow cytometry, expression of the Fas antigen on lymphocytes of 23 HIV infected individuals (CDC category 2, CD4 200-499 cells/microL, n = 10; CDC category 3, CD4 < 200 cells/microL, n = 13) and 10 healthy controls was examined. Both CD3+CD4+ and CD3+CD8+ subsets were examined for their expression of this marker. In lymphocytes of healthy controls, 47% of the CD3+CD4+ and 45% of the CD3+CD8+ cells were Fas antigen positive. This percentage was significantly increased in CD4 cells from HIV infected patients belonging to CDC category 3, but was unchanged from normal values in CDC category 2 subjects. The increase in the percentage of CD4+ T cells expressing Fas antigen in patients correlated significantly with the decrease in circulating CD4 T cell count (P < 0.009). In addition, by examining mean fluorescence intensity, we found that the amount of Fas expression per cell was increased threefold in CD3+CD4+ cells and increased twofold in CD3+CD8+ cells in category 3 patients. These results demonstrate that an increase in Fas antigen expression occurs during HIV infection.