Characterization of a variant of human T-lymphotropic virus type I isolated from a healthy member of a remote, recently contacted group in Papua New Guinea.
Characterization of a variant of human T-lymphotropic virus type I isolated from a healthy member of a remote, recently contacted group in Papua New Guinea.
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从巴布亚新几内亚一个偏远、最近接触过的群体的健康成员中分离出 I 型人类 T 淋巴细胞病毒变种的特征。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
D. Gajdusek
中科院分区：
文献类型：
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作者：
R. Yanagihara;V. Nerurkar;R. Garruto;M. Miller;M. Leon;C. L. Jenkins;R. Sanders;P. Liberski;M. Alpers;D. Gajdusek
我们报告了一种人类嗜T淋巴细胞病毒I型（HTLV-I）的变异体的特征，该病毒分离自一种白细胞介素2依赖性、CD 8 + T细胞系，该细胞系来源于一个偏远的、最近与巴布亚新几内亚马当省的狩猎园艺家群体（Hagahai）的健康成员的外周血单核细胞。通过免疫荧光对该变体（命名为PNG-1）进行抗原性表征，使用针对HTLV-I gag基因产物的单克隆和多克隆抗体，表明gag编码的蛋白质p19和p24（即使在与5-溴-2 '-脱氧尿苷孵育后）也没有表达。使用血清学和/或病毒学证实的HTLV-I脊髓神经病患者血清、HTLV-I感染兔血清和针对主要包膜糖蛋白gp 46的C末端制备的抗体，通过Western印迹分析证明了15、19、46、53和61/68 kDa的病毒特异性蛋白。PNG-1前病毒DNA的限制性内切酶图谱与HTLV-I（MT-2）的原型株不同，但聚合酶链反应证实，PNG-1肯定是HTLV-I，而不是HTLV-II。核苷酸测序和进一步的分子遗传学研究，这种变异可能会提供深入了解HTLV-I的起源和进化。
We report the characterization of a variant of human T-lymphotropic virus type I (HTLV-I) isolated from an interleukin 2-dependent, CD8+ T-cell line derived from peripheral blood mononuclear cells of a healthy member of a remote, recently contacted hunter-horticulturalist group (Hagahai) in Madang province of Papua New Guinea. Antigenic characterization of this variant, designated PNG-1, by immunofluorescence, indicated no expression of gag-encoded proteins p19 and p24 (even after incubation with 5-bromo-2'-deoxyuridine), using monoclonal and polyclonal antibodies against HTLV-I gag gene products. Virus-specific proteins of 15, 19, 46, 53, and 61/68 kDa were demonstrated by Western blot analysis, using sera from patients with serologically and/or virologically confirmed HTLV-I myeloneuropathy, sera from HTLV-I-infected rabbits, and antibodies prepared against the C terminus of the major envelope glycoprotein gp46. Restriction endonuclease maps of PNG-1 proviral DNA differed from that of a prototype strain of HTLV-I (MT-2), but, as verified by polymerase chain reaction, PNG-1 was definitely HTLV-I, not HTLV-II. Nucleotide sequencing and further molecular genetic studies of this variant may provide insights into the origin and evolution of HTLV-I.