The b-ZIP transcription factor FgTfmI is required for the fungicide phenamacril tolerance and pathogenecity in Fusarium graminearum
The b-ZIP transcription factor FgTfmI is required for the fungicide phenamacril tolerance and pathogenecity in Fusarium graminearum
复制标题
b-ZIP 转录因子 FgTfmI 是禾谷镰刀菌对杀菌剂非那丙烯利的耐受性和致病性所必需的
DOI：
10.1002/ps.5454
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发表时间：
2019-12-01
影响因子：
4.1
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
--
作者：
Liu, Na;Wu, Siqi;Ma, Zhonghua
镰刀菌头枯病（Fusarium head blight，FHB）是一种主要由禾谷镰刀菌（Fusarium graminearum）引起的世界性禾谷类作物病害。由于抗赤霉病小麦品种的缺乏，化学杀菌剂是目前防治赤霉病最有效的方法。在过去的几年里，一种新型的氰基丙烯酸酯杀菌剂，非那普利，已被广泛应用于中国的FHB疾病的管理。在以前的研究中，我们确定了肌球蛋白I（FgMyo 1）是phenamacril的目标，是必不可少的真菌毒素脱氧雪腐镰刀菌烯醇（DON）的生物合成和真菌生长。然而，FgMYO 1基因表达的调控在很大程度上仍然是未知的。在这项研究中，我们确定了一个b-ZIP转录因子，FgTfmI，它调节非那普利治疗后FgMYO 1的mRNA表达。FgTfmI直接与FgMYO 1的启动子区结合，并且是响应于非那普利治疗而上调FgMYO 1所需的。FgTfmI缺失突变体（Delta FgTfmI）表现出轻微的生长缺陷，但对苯那普利表现出超敏反应，而对其他测试的杀菌剂不敏感。结论FgTfmI在调控菲那普利抗性基因、DON合成基因和致病基因的转录过程中起重要作用。禾谷早熟禾(c)2019化学工业协会
BACKGROUNDFusarium head blight (FHB) is a devastating disease of cereal crops worldwide mainly caused by Fusarium graminearum. Due to the unavailability of FHB-resistant wheat cultivars, chemical fungicide application is currently the most effective approach for controlling FHB now. In the last few years, a novel cyanoacrylate fungicide, phenamacril, has been widely used in China for FHB disease management. In previous studies, we identified that myosin I (FgMyo1) is the target of phenamacril and is essential for mycotoxin deoxynivalenol (DON) biosynthesis and fungal growth. However, the regulation of FgMYO1 gene expression is still largely unknown.RESULTSIn this study, we identified a b-ZIP transcription factor, FgTfmI, which regulates the mRNA expression of FgMYO1 upon phenamacril treatment. The FgTfmI directly binds to the promoter region of FgMYO1, and is required for the upregulation of FgMYO1 in response to phenamacril treatment. The deletion mutant of FgTFMI (Delta FgTfmI) displayed a slight growth defect, while it showed hypersensitivity to phenamacril, but not to other tested fungicides. FgTfmI also contributed to DON biosynthesis and the infection process in planta.CONCLUSIONSThe transcription factor FgTfmI plays an important role in regulating transcription of the genes involved in phenamacril tolerance, DON biosynthesis and virulence in F. graminearum. (c) 2019 Society of Chemical Industry