Intraganglionic interactions between satellite cells and adult sensory neurons
Intraganglionic interactions between satellite cells and adult sensory neurons
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DOI：
10.1016/j.mcn.2015.05.001
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发表时间：
2015-07-01
影响因子：
3.5
通讯作者：
Zochodne, Douglas W.
中科院分区：
文献类型：
--
作者：
Christie, Kimberly;Koshy, Dilip;Zochodne, Douglas W.
神经元周卫星细胞与感觉神经元有着密切的解剖学关系，这表明它们在功能上有着密切的合作和相互支持。我们在成人感觉背根神经节（DRG）中研究了这种关系的几个方面。合作包括支持过程的卫星细胞集群，诱导远端分支行为的索马信号，卫星细胞的能力，以响应其邻近神经元的远端轴突损伤的支持，以及直接的神经元-卫星细胞交换的证据。在体外，紧密贴壁共收获的卫星细胞通常聚集在新的突起周围，并且卫星细胞组吸引神经突突起。在体内完整的DRG内完整的感觉神经元的假单极过程中也遇到了类似的聚类。虽然短期暴露于瞬时梯度的PTEN抑制剂的成年感觉神经元的远端生长锥对其行为的影响可以忽略不计，但暴露于索马诱导其远端神经突和分支的早期和实质性生长，这是局部索马信号传导的一个实例。反过来，当远端神经元轴突受到损伤时，卫星细胞通过扩大和增殖来感知。我们还观察到，卫星细胞能够内化和表达的神经元荧光标记，diamidino黄，远程应用于远端损伤的轴突的神经元和逆行运输到背根神经节感觉神经元。这些发现说明了神经节内神经元和神经胶质细胞之间的强大相互作用，涉及双向信号，这些特征可能对再生反应和持续维护至关重要。(C)由Elsevier Inc.出版。
Perineuronal satellite cells have an intimate anatomical relationship with sensory neurons that suggests close functional collaboration and mutual support. We examined several facets of this relationship in adult sensory dorsal root ganglia (DRG). Collaboration included the support of process outgrowth by clustering of satellite cells, induction of distal branching behavior by soma signaling, the capacity of satellite cells to respond to distal axon injury of its neighboring neurons, and evidence of direct neuron-satellite cell exchange. In vitro, closely adherent coharvested satellite cells routinely clustered around new outgrowing processes and groups of satellite cells attracted neurite processes. Similar clustering was encountered in the pseudounipolar processes of intact sensory neurons within intact DRG in vivo. While short term exposure of distal growth cones of unselected adult sensory neurons to transient gradients of a PTEN inhibitor had negligible impacts on their behavior, exposure of the soma induced early and substantial growth of their distant neurites and branches, an example of local soma signaling. In turn, satellite cells sensed when distal neuronal axons were injured by enlarging and proliferating. We also observed that satellite cells were capable of internalizing and expressing a neuron fluorochrome label, diamidino yellow, applied remotely to distal injured axons of the neuron and retrogradely transported to dorsal root ganglia sensory neurons. The findings illustrate a robust interaction between intranganglionic neurons and glial cells that involve two way signals, features that may be critical for both regenerative responses and ongoing maintenance. (C) 2015 Published by Elsevier Inc.