AGING INFLUENCES ADAPTATIONS OF THE NEUROMUSCULAR JUNCTION TO ENDURANCE TRAINING
AGING INFLUENCES ADAPTATIONS OF THE NEUROMUSCULAR JUNCTION TO ENDURANCE TRAINING
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DOI：
10.1016/j.neuroscience.2011.05.070
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发表时间：
2011-09-05
期刊：
影响因子：
3.3
通讯作者：
Glass, E. K.
中科院分区：
文献类型：
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作者：
Deschenes, M. R.;Roby, M. A.;Glass, E. K.
本研究旨在确定是否老化影响神经肌肉接头（NMJ）的适应运动训练。将20只年轻成年大鼠（8个月）和20只老年大鼠（24个月）分配到跑步机运动或久坐状态。在10周的实验期后，将大鼠安乐死，并取出比目鱼肌和跖肌并冷冻。对肌肉的纵向切片进行荧光染色，以观察慢肌纤维和快肌纤维上的突触前神经末梢和突触后终板。用共聚焦显微镜收集图像并定量。对肌肉横截面进行组织化学染色，以评估肌纤维分布（大小和纤维类型）。我们对NMJ的分析揭示了对衰老、运动训练及其相互作用的高度特异性和敏感性。在比目鱼肌中，慢收缩型NMJs表现出显著性差异（P
This investigation sought to determine if aging affected adaptations of the neuromuscular junction (NMJ) to exercise training. Twenty young adult (8 months) and 20 aged (24 months) rats were assigned to either a program of treadmill exercise, or sedentary conditions. Following the 10-week experimental period, rats were euthanized, and soleus and plantaris muscles were removed and frozen. Longitudinal sections of the muscles were fluorescently stained to visualize pre-synaptic nerve terminals and post-synaptic endplates on both slow- and fast-twitch fibers. Images were collected with confocal microscopy and quantified. Muscle cross-sections were histochemically stained to assess muscle fiber profiles (size and fiber type). Our analysis of NMJs revealed a high degree of specificity and sensitivity to aging, exercise training, and their interaction. In the soleus, slow-twitch NMJs demonstrated significant (P