Use of alkaline phosphatase staining to differentiate canine osteosarcoma from other vimentin-positive tumors
Use of alkaline phosphatase staining to differentiate canine osteosarcoma from other vimentin-positive tumors
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DOI：
10.1354/vp.42-2-161
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发表时间：
2005-03-01
影响因子：
2.4
通讯作者：
Hoffmann, W
中科院分区：
文献类型：
--
作者：
Barger, A;Graca, R;Hoffmann, W
溶解性骨病变的穿刺是一种很好的诊断原发性骨瘤的初步评估方法。然而，细胞学上很难将骨肉瘤（OSA）与其他骨肿瘤（包括纤维肉瘤、软骨肉瘤、滑膜细胞肉瘤和浆细胞骨髓瘤）区分开来。本研究的目的是通过免疫细胞化学或免疫组织化学检测碱性磷酸酶（ALP）染色区分OSA与其他表达vimentin的肿瘤的敏感性和特异性。ALP是一种存在于多种组织中的水解酶，包括肝、肾、肠、胎盘和骨。假设，积极生成骨的肿瘤应该是ALP染色特异性阳性的。未染色的细胞学标本与硝基蓝四氮氯化铵/5-溴-no-4-氯-3-吲哚基磷酸甲苯胺盐磷酸酶底物孵育8-10分钟。阳性反应使细胞膜呈灰色或黑色。用罗曼诺夫斯基染色法反染样品以确定样品是否具有代表性细胞。共有61例vimentin阳性肿瘤进行了评估并经组织病理学证实。表达vimentin且ALP阳性的肿瘤包括33例osa、1例骨多小叶肿瘤、1例无色素黑色素瘤和1例软骨肉瘤。表达vimentin且ALP阴性的肿瘤包括软骨肉瘤（四分之三）、多发性纤维肉瘤和多发性滑膜细胞肉瘤。灵敏度为100%，特异性为89%。综上所述，ALP在OSA的诊断中是一个高度敏感且具有相当特异性的标志物。
Aspiration of lytic bone lesions is an excellent diagnostic test in the initial evaluation of primary bone neoplasia. However, cytologically, it can be difficult to differentiate osteosarcoma (OSA) from other bone neoplasms, including fibrosarcoma, chondrosarcoma, synovial cell sarcoma, and plasma cell myeloma. The purpose of this study is to determine the sensitivity and specificity of alkaline phosphatase (ALP) staining to differentiate OSA from other tumors that express vimentin by immunocytochemistry or immunohistochemistry. ALP is a hydrolytic enzyme present in multiple tissues including liver, kidney, intestine, placenta, and bone. Hypothetically, neoplasms actively producing bone should be specifically positive for ALP staining. Unstained, cytologic specimens were incubated for 8-10 minutes with nitroblue tetrazolium chloride/5-broi-no-4-chloro-3-indolyl phosphate toluidine salt-phosphatase Substrate. A positive reaction stains the membrane of the cells gray to black. Samples were counterstained with a Romanowsky's stain to determine whether the sample was of representative cellularity. A total of 61 vimentin-positive neoplasms have been evaluated and confirmed histopathologically. Tumors that expressed vimentin and were positive for ALP included 33 OSAs, one multilobular tumor of bone, one amelanotic melanoma, and one chondrosarcoma. Tumors that expressed vimentin and were negative for ALP included chondrosarcomas (three of four), Multiple fibrosarcomas, and multiple synovial cell sarcomas. The sensitivity is 100%, and the specificity is 89%. In conclusion, ALP appears to be a highly sensitive and fairly specific marker in the diagnosis of OSA.