A Self‐Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High‐Resolution Structural Detail
A Self‐Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High‐Resolution Structural Detail
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具有可调腔尺寸和高分辨率结构细节的自组装菱面体 DNA 晶体支架
DOI：
10.1002/anie.202005505
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yan, Hao
中科院分区：
文献类型：
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作者：
Simmons, Chad R.;MacCulloch, Tara;Zhang, Fei;Liu, Yan;Stephanopoulos, Nicholas;Yan, Hao
DNA是用于构建具有可调性质的3D晶体的理想分子，这是由于其基于规范沃森-克里克碱基配对的高度可编程性，并且通过固定的霍利迪结和粘性末端内聚促进了所有三维的晶体组装。尽管这些系统的承诺，只有少数独特的晶体支架已被报道。在此，我们描述了一种新的晶体系统，其具有通过一系列与互补粘性末端连接在一起的霍利迪连接来介导3D支架组装的重复序列。通过使用优化的连接序列，我们可以确定包含R3晶体对称性的高分辨率（2.7 μ m）结构，随后使用修改的粘性末端序列略有改善（2.6 μ m）。 固定的霍利迪结序列使我们能够制造出提供前所未有的原子细节的晶体。此外，我们通过在结之间添加额外的螺旋圈将晶体腔扩大了50%，并通过分子置换将结构解到4.5 μ m分辨率。
DNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson–Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high‐resolution (2.7 Å) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 Å) using a modified sticky‐end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 Å resolution by molecular replacement.