Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals
Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals
复制标题
在所有血清反应呈阳性、有症状和无症状的个体中均检测到 1 型人类免疫缺陷病毒
DOI：
10.1128/jcm.28.1.16-19.1990
复制
发表时间：
1990
影响因子：
9.4
通讯作者：
H H Balfour
中科院分区：
文献类型：
--
作者：
J B Jackson;S. Kwok;J. Sninsky;J. S. Hopsicker;K. Sannerud;F. Rhame;K. Henry;M. Simpson;H H Balfour
Between February 1987 and October 1988, peripheral mononuclear blood cells (PBMC) from 409 adult individuals antibody positive by Western (immuno-)blot for human immunodeficiency virus type 1 (HIV-1) (56 acquired immunodeficiency syndrome [AIDS] patients, 88 patients with AIDS-related complex, and 265 asymptomatic individuals) were consecutively cultured for HIV-1 or tested for the presence of HIV-1 DNA sequences by a polymerase chain reaction assay (PCR). We isolated HIV-1 or detected HIV-1 DNA sequences from the PBMC of all 409 HIV-1 antibody-positive individuals. None of 131 healthy HIV-1 antibody-negative individuals were HIV-1 culture positive, nor were HIV-1 DNA sequences detected by PCR in the blood specimens of 43 seronegative individuals. In addition, HIV-1 PCR and HIV-1 culture were compared in testing the PBMC of 59 HIV-1 antibody-positive and 20 HIV-1 antibody-negative hemophiliacs. Both methods were found to have sensitivities and specificities of at least 97 and 100%, respectively. In contrast, the sensitivities of serum HIV-1 antigen testing in AIDS patients and asymptomatic seropositive patients were 42 and 17%, respectively. Our ability to directly demonstrate HIV-1 infection in all HIV-1 antibody-positive individuals provides definitive support that HIV-1 antibody positivity is associated with present HIV-1 infection. Moreover, the sensitivities and specificities of PCR and culture for the detection of HIV-1 appear to be equivalent, and both methods are superior to testing for HIV-1 antigen in serum for the direct detection of HIV-1.
携带艾滋病毒抗体的血友病患者处于活动性感染状态。
DOI：
--
发表时间：
1988
期刊：
JAMA : the journal of the American Medical Association
影响因子：
--
作者：
Jackson,JB;Sannerud,KJ;Hopsicker,JS;Kwok,SY;Edson,JR;BalfourJr,HH
通讯作者：
BalfourJr,HH
逆转录病毒作为致癌物和病原体：期望和现实。
影响因子：
11.2
作者：
Duesberg,PH
通讯作者：
Duesberg,PH