Catecholamine levels and turnover during aging in brain regions of male C57BL/6J mice
Catecholamine levels and turnover during aging in brain regions of male C57BL/6J mice
复制标题
雄性 C57BL/6J 小鼠大脑区域衰老过程中的儿茶酚胺水平和周转率
DOI：
10.1016/0006-8993(81)90864-7
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Caleb E. Finch
中科院分区：
文献类型：
--
作者：
H. Osterburg;H. G. Donahue;J. A. Severson;Caleb E. Finch
A radioenzymatic assay is described for measuring brain catecholamines (CA) and 3,4-dihydroxyphenylacetic acid (DOPAC) in the same tissue extract. The [3H]-methylated products are differentially extracted and then acetylated by acetic anhydride, followed by thin layer chromatography in non-basic solvents. Routine sensitivity is 3–5 pg per sample. This assay gave CA levels in brain regions which generally agreed with previous reports.CA levels/mg protein and turnover (after injection ofα-methyl-p-tyrosine) were measured in male C57BL/6J mice at various ages between 4 and 30 months, the average lifespan of male C57BL/6J mice. No region showed progressive age changes in levels or turnover, or changes before midlife, 8–12 months. Brain regions with no evidence of age changes include globus pallidus, zona incerta, substantia nigra, cerebellum, and olfactory bulbs. Small changes (10–25%) were detected in only some regions of mice aged ≥ 24 months; few changes were statistically significant. CA levels and turnover decreased in some samplings of striatum and median eminence-arcuate nucleus. In contrast, DA levels increased in anterior pituitary and possibility in medial preoptic region. DOPAC levels (measured only in rostral striatum) decreased by 20% at 28 months. These results diverge from the larger, progressive decreases of DA reported in human striatum during aging.
