SEQUENCE REQUIREMENTS FOR NUCLEAR LOCATION OF SIMIAN VIRUS-40 LARGE-T-ANTIGEN
SEQUENCE REQUIREMENTS FOR NUCLEAR LOCATION OF SIMIAN VIRUS-40 LARGE-T-ANTIGEN
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DOI：
10.1038/311033a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
SMITH, AE
中科院分区：
文献类型：
--
作者：
KALDERON, D;RICHARDSON, WD;SMITH, AE
SV-40大T基因中的点突变（由混合寡核苷酸诱变产生并导致Lys 128转化为Thr）产生了在细胞质[大鼠]而非细胞核中检测到的大T抗原。周围序列Lys-128 Lys-Lys-Arg-Lys-Val-Glu内的缺失也产生细胞质大T，并定义了参与核定位的蛋白质区域。
A point mutation in the SV-40 large-T gene, which was generated by mixed oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a large-T antigen that was detected in the cytoplasm [rat] but not the nucleus of cells. Deletions within the surrounding sequence Lys-128Lys-Lys-Arg-Lys-Val-Glu also produce cytoplasmic large-T and define a region of the protein involved in nuclear location.