Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in Saccharomyces cerevisiae.
Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in Saccharomyces cerevisiae.
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DNA 修复在甲磺酸乙酯诱导的酿酒酵母诱变中的作用。
DOI：
10.1093/carcin/3.4.439
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发表时间：
1982
期刊：
影响因子：
4.7
通讯作者：
Higgins,D
中科院分区：
文献类型：
--
作者：
Prakash,L;Higgins,D
甲磺酸乙酯 (EMS) 诱导的两个位点（通过 GC 到 AT 转换优先恢复），cycl-131 和 cycl-115，已在属于 rad52 上位组的酵母的读敏感、rad、突变体中进行了检查。当稳定期二倍体细胞用 EMS 处理时，rad50、rad51、rad52、rad54 和 rad56 突变体显示两个测试位点的回复减少。 EMS 诱导的回复和 EMS 诱导的同源有丝分裂基因内重组之间没有发现相关性。 EMS处理后rad6rad52双突变体的存活表明存在一个上位基团用于修复酵母中EMS诱导的致死损伤。提出了一个涉及特定病变的误修复诱变的模型来解释实验结果。
Ethyl methanesulfonate (EMS)-induced reversion of two sites which revert preferentially by GC to AT transitions,cycl-131andcycl-115, has been examined in readiation sensitive, rad, mutants of yeast belonging to the rad52 epistasis group. Therad50,rad51,rad52,rad54andrad56mutants showed reduced reversion of both tester sites when stationary phase diploid cells were treated with EMS. No correlation was found between EMS-induced reversion and EMS-induced homologous mitotic intragenic recombination. Survival ofrad6rad52double mutants following EMS treatment indicates that there is one epistasis group for the repair of EMS-induced lethal damage in yeast. A model involving misrepair mutagenesis of specific lesions is proposed to account for the experimental results.