A monophasic extraction strategy for the simultaneous lipidome analysis of polar and nonpolar retina lipids[S]
A monophasic extraction strategy for the simultaneous lipidome analysis of polar and nonpolar retina lipids[S]
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极性和非极性视网膜脂质同步脂质组分析的单相提取策略[S]
DOI：
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发表时间：
2014
影响因子：
6.5
通讯作者：
G. E. Reid
中科院分区：
文献类型：
--
作者：
Todd A. Lydic;J. Busik;G. E. Reid
使用双相氯仿/甲醇/水混合物的脂质提取，加上官能团选择性衍生和直接输注纳米ESI-高分辨率/准确的MS，有利于同时分析来自单个视网膜脂质提取物的高极性和非极性脂质，包括低丰度高极性神经节苷脂脂质，非极性鞘脂和丰富的甘油磷脂。定量比较表明，双相脂质提取方法产生了类似的脂质分布，从建立的“金标准”的双相脂质提取方法，已知丰富的高极性神经节苷脂或非极性脂质，分别只有适度的相对离子抑制作用。因此，这种改进的脂质提取和分析策略使得能够从最少量的生物样品对广泛极性和丰度范围内的脂质种类进行详细的脂质组分析，并且在分析之前不需要多个脂质类别特异性提取或色谱分离。
Lipid extraction using a monophasic chloroform/methanol/water mixture, coupled with functional group selective derivatization and direct infusion nano-ESI-high-resolution/accurate MS, is shown to facilitate the simultaneous analysis of both highly polar and nonpolar lipids from a single retina lipid extract, including low abundance highly polar ganglioside lipids, nonpolar sphingolipids, and abundant glycerophospholipids. Quantitative comparison showed that the monophasic lipid extraction method yielded similar lipid distributions to those obtained from established “gold standard” biphasic lipid extraction methods known to enrich for either highly polar gangliosides or nonpolar lipids, respectively, with only modest relative ion suppression effects. This improved lipid extraction and analysis strategy therefore enables detailed lipidome analyses of lipid species across a broad range of polarities and abundances, from minimal amounts of biological samples and without need for multiple lipid class-specific extractions or chromatographic separation prior to analysis.
稳定同位素辅助脂质组学与非靶向同位素过滤相结合，是揭示脂质代谢复杂动力学的工具
影响因子：
7.4
作者：
Li, Jia;Hoene, Miriam;Xu, Guowang
通讯作者：
Xu, Guowang
影响因子：
20.3
作者：
Nimrichter, Leonardo;Burdick, Monica M.;Schnaar, Ronald L.
通讯作者：
Schnaar, Ronald L.