Induction of Diverse Bioactive Secondary Metabolites from the Mangrove Endophytic Fungus Trichoderma sp. (Strain 307) by Co-Cultivation with Acinetobacter johnsonii (Strain B2).
Induction of Diverse Bioactive Secondary Metabolites from the Mangrove Endophytic Fungus Trichoderma sp. (Strain 307) by Co-Cultivation with Acinetobacter johnsonii (Strain B2).
复制标题
通过与约氏不动杆菌（菌株 B2）共培养，从红树林内生真菌木霉菌（菌株 307）中诱导多种生物活性次生代谢物
DOI：
10.3390/md15020035
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发表时间：
2017-02-10
期刊：
影响因子：
5.4
通讯作者：
Liu L
中科院分区：
文献类型：
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作者：
Zhang L;Niaz SI;Khan D;Wang Z;Zhu Y;Zhou H;Lin Y;Li J;Liu L
两种新的倍半萜，microsphaeropsisin B（1）和C（2），和两种新的脱-O-甲基lasiodiplodin，（3R，7 R）-7-羟基-脱-O-甲基lasiodiplodin（4）和（3R）-5-氧代-脱-O-甲基lasiodiplodin（5），以及一种新的天然产物（6）和十二种已知化合物（3，7-17），从红树林内生真菌Trichoderma sp. 307和水生病原细菌Acinetobacter johnsonii B2共培养物中分离得到一株内生真菌Trichoderma sp.它们的结构，包括绝对构型，阐明了广泛的分析光谱数据，电子圆二色性，Mo 2（AcO）4诱导的圆二色性，并与报道的数据进行比较。对分离得到的化合物进行了α-葡萄糖苷酶抑制活性和细胞毒性试验。新化合物4和5显示出有效的α-葡萄糖苷酶抑制活性，IC 50值分别为25.8和54.6 µM，比阳性对照（阿卡波糖，IC 50 = 703.8 µM）更有效。良好的生物活性测试结果使我们能够探索毛双螺素中的α-葡萄糖苷酶抑制剂。
Two new sesquiterpenes, microsphaeropsisin B (1) and C (2), and two new de-O-methyllasiodiplodins, (3R, 7R)-7-hydroxy-de-O-methyllasiodiplodin (4) and (3R)-5-oxo-de-O-methyllasiodiplodin (5), together with one new natural product (6) and twelve known compounds (3, 7–17), were isolated from the co-cultivation of mangrove endophytic fungus Trichoderma sp. 307 and aquatic pathogenic bacterium Acinetobacter johnsonii B2. Their structures, including absolute configurations, were elucidated by extensive analysis of spectroscopic data, electronic circular dichroism, Mo2(AcO)4-induced circular dichroism, and comparison with reported data. All of the isolated compounds were tested for their α-glucosidase inhibitory activity and cytotoxicity. New compounds 4 and 5 exhibited potent α-glucosidase inhibitory activity with IC50 values of 25.8 and 54.6 µM, respectively, which were more potent than the positive control (acarbose, IC50 = 703.8 µM). The good results of the tested bioactivity allowed us to explore α-glucosidase inhibitors in lasiodiplodins.