Essential role of the posterior morphogen nanos for germline development in Drosophila
Essential role of the posterior morphogen nanos for germline development in Drosophila
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DOI：
10.1038/380708a0
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发表时间：
1996-04-25
期刊：
影响因子：
64.8
通讯作者：
Kitamura, T
中科院分区：
文献类型：
--
作者：
Kobayashi, S;Yamada, M;Kitamura, T
在许多动物群体中，种系形成所需的因子位于种质(1)（卵细胞质的一个区域）中。在果蝇胚胎中，种质位于后极区域，并在极细胞（种系祖细胞）中遗传。移植实验证明，种质中含有形成种系的因子(2-4)，种质还直接指导腹部的形成(5)。遗传分析表明，有一个共同机制将腹部和种系形成因子定位到后极(6-12)。腹部形成的关键因素是纳米（nos）蛋白（nanos）(13-15)。在这里，我们证明 nos 对于果蝇种系形成也是必需的。缺乏纳米活性的极细胞无法迁移到性腺，因此不会成为功能性生殖细胞。在这种极细胞中，通常在性腺内启动的基因表达在极细胞迁移过程中过早开始。种系前体基因的过早激活可能意味着这些细胞无法正常发育。 nos 蛋白在果蝇种系形成中的功能与其在其他动物中与种质相关的观察结果是一致的(16-18)。
IN many animal groups, factors required for germline formation are localized in germ plasm(1), a region of the egg cytoplasm, In Drosophila embryos, germ plasm is located in the posterior pole region and is inherited in pole cells, the germline progenitors. Transplantation experiments have demonstrated that germ plasm contains factors that can form germline(2-4), and germ plasm also directs abdomen formation(5). Genetic analysis has shown that a common mechanism directs the localization of the abdomen and germline-forming factors to the posterior pole(6-12). The critical factor for abdomen formation is the nanos (nos) protein (nanos)(13-15). Here we show that nos is also essential for germline formation in Drosophila; pole cells lacking nanos activity fail to migrate into the gonads, and so do not become functional germ cells. In such pole cells, gene expression, which normally initiates within the gonad, begins prematurely during pole cell migration. Premature activation of genes in germline precursors may mean that these cells fail to develop normally. A function for nos protein in Drosophila germline formation is compatible with observations of its association with germ plasm in other animals(16-18).