Reversal of docosahexaenoic acid deficiency in the rat brain, retina, liver, and serum.
Reversal of docosahexaenoic acid deficiency in the rat brain, retina, liver, and serum.
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发表时间：
2001-03
影响因子：
6.5
通讯作者：
T. Moriguchi;James D. Loewke;M. Garrison;J. Catalan;N. Salem
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文献类型：
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作者：
T. Moriguchi;James D. Loewke;M. Garrison;J. Catalan;N. Salem
在实验动物以及食用植物油配方奶粉的人类婴儿中，视网膜或大脑中二十二碳六烯酸 (DHA) 的损失与神经系统功能的损失有关。在年轻成年大鼠中研究了 DHA 损失的可逆性以及 n-6 二十二碳五烯酸 (DPAn-6) 增加的补偿。 Long-Evans 大鼠在两代中都处于非常低水平的 n-3 脂肪酸之下。 F2代、n-3缺乏的动物在7周龄时被提供含有α-亚麻酸和DHA的补充饮食。另一组F2代大鼠维持相同成分的n-3-充足饮食。在第0、1、2、4和8周时从两组动物收集大脑、视网膜、肝脏和血清组织。测定了每个组织的 DHA、DPAn-6 和其他脂肪酸的浓度，并测定了恢复率和完成 DHA 恢复所需的时间长度。饮食逆转后 1 周和 2 周时，大脑中的 DHA 水平仅部分恢复，分别上升至 n-3 充足组水平的约 20% 和 35%。直到开始补充饮食后 8 周才完全恢复。尽管视网膜 DHA 累积的初始速率高于大脑 DHA，但 DHA 恢复的半衰期仅稍长一些。另一方面，在饮食逆转的两周内，血清和肝脏中的 DHA 水平分别大约被替换了 90% 和 100%。与肝脏和循环系统相比，神经系统中 DHA 补充的总量和时间进程表明，运输相关过程可能会限制视网膜和大脑中 DHA 的补充率。-- Moriguchi, T., J. Loewke, M. Garrison, J. N. Catalan, N. Salem, Jr. 逆转大鼠大脑、视网膜、肝脏和血清中二十二碳六烯酸缺乏症。 J.脂质研究。 2001。42：419--427。
The loss of docosahexaenoic acid (DHA) from the retina or brain has been associated with a loss in nervous-system function in experimental animals, as well as in human infants fed vegetable oil-based formulas. The reversibility of the loss of DHA and the compensation by an increase in the n-6 docosapentaenoic acid (DPAn-6) was studied in young adult rats. Long-Evans rats were subjected to a very low level of n-3 fatty acids through two generations. The F2 generation, n-3-deficient animals at 7 weeks of age were provided a repletion diet containing both alpha-linolenate and DHA. A separate group of F2 generation rats had been maintained on an n-3-adequate diet of the same composition. Tissues from the brain, retina, liver, and serum were collected on weeks 0, 1, 2, 4, and 8 from both groups of animals. The concentrations of DHA, DPAn-6, and other fatty acids were determined and the rate of recovery and length of time needed to complete DHA recovery were determined for each tissue. The DHA level in the brain at 1 and 2 weeks after diet reversal was only partially recovered, rising to approximately 20% and 35%, respectively, of the n-3-adequate group level. Full recovery was not obtained until 8 weeks after initiation of the repletion diet. Although the initial rate of retinal DHA accretion was greater than that of brain DHA, the half-time for DHA recovery was only marginally greater. On the other hand, the levels of DHA in the serum and liver were approximately 90% and 100% replaced, respectively, within 2 weeks of diet reversal. A consideration of the total amounts and time courses of DHA repleted in the nervous system compared with the liver and circulation suggests that transport-related processes may limit the rate of DHA repletion in the retina and brain.-- Moriguchi, T., J. Loewke, M. Garrison, J. N. Catalan, N. Salem, Jr. Reversal of docosahexaenoic acid deficiency in the rat brain, retina, liver, and serum. J. Lipid Res. 2001. 42: 419--427.