Levels of amino acids and related compounds in bronchoalveolar lavage fluids of asthmatic patients
Levels of amino acids and related compounds in bronchoalveolar lavage fluids of asthmatic patients
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DOI：
10.1164/ajrccm.155.2.9032174
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发表时间：
1997-02-01
影响因子：
24.7
通讯作者：
Vogel, WH
中科院分区：
文献类型：
--
作者：
Hofford, JM;Milakofsky, L;Vogel, WH
支气管肺泡灌洗 (BAL) 液的成分已被证明可以反映多种肺部疾病的存在及其可能的病因。目前，虽然研究报告了支气管肺泡灌洗液中细胞因子和化合物（例如主要碱性蛋白）的浓度，但只有细胞成分、总蛋白、白蛋白和免疫球蛋白得到了明确的定义。我们假设这一点。氨基酸和相关的氨基化合物是生理酸生化过程中众所周知的参与者，存在于支气管肺泡灌洗液中，可能与哮喘有关。我们的目标是通过测量正常对照受试者和哮喘患者中的这些氨基化合物来扩展对人类 BAL 液体的总化学特征和临床价值的了解。高压液相色谱分析显示存在 25 种化合物。发现对照受试者和患者中的少数化合物的值 > 1.0 nmol/ml，而大多数化合物的浓度相对较低，< 1.0 nmol/ml。哮喘患者体内的天冬酰胺、磷酸乙醇胺和牛磺酸显着增加。我们得出的结论是，BAL 液中氨基酸和相关氨基化合物的现状可作为研究各种肺部疾病的潜在诊断工具。天冬酰胺、磷酸乙醇胺和牛磺酸增加对哮喘患者的意义进行了讨论，值得进一步研究。
The constituents of bronchoalveolar lavage (BAL) fluid have been shown to reflect the presence and possible etiology of several pulmonary diseases. Presently, although research studies have reported the concentrations of cytokines and compounds such as major basic protein in BAL fluids, only the cellular elements, total protein, albumin, and immunoglobulins have been well defined. We hypothesize that. amino acids and related amino compounds, well known participants in physiologic acid biochemical processes, are present in BAL fluid and may have involvement in asthma. Our objective was to extend knowledge of the total chemical profile and clinical value of BAL fluids In humans by measuring these amino compounds in normal control subjects and asthmatic patients, Analysis by high-pressure liquid chromatography revealed the presence of 25 compounds. A few compounds in control subjects and patients were found to have values > 1.0 nmol/ml, while the majority were present in comparatively low concentrations < 1.0 nmol/ml. Asparagine, phosphoethanolamine, and taurine were significantly increased in the asthmatic patients. We conclude that the present profile of amino acids and related amino compounds in BAL fluid serves as a potential diagnostic tool in the study of various pulmonary disorders. The significance of increased asparagine, phosphoethanolamine, and taurine in the asthmatic patients is discussed and deserves further study.