Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase.
Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase.
复制标题
人嘌呤霉素敏感氨肽酶基因的克隆和分析。
DOI：
10.1006/bbrc.1999.0604
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发表时间：
1999
影响因子：
3.1
通讯作者：
Hersh,LB
中科院分区：
文献类型：
--
作者：
Thompson,MW;Tobler,A;Fontana,A;Hersh,LB
编码人嘌呤霉素敏感氨基肽酶(PSA)的基因已被克隆和鉴定。人PSA基因由23个外显子和22个内含子组成，在17q12-21之间跨越了约40kb的17号染色体。对人PSA转录本5‘端的分析表明，根据RT-PCR、5’RACE和表达序列标签的计算机分析，翻译起始点对应于人PSAcDNA的第210个核苷酸。人PSA基因和人氨基肽酶N(APN)基因外显子/外显子边界的比较显示几乎没有保守性，这表明这两个基因在蛋白质序列上密切相关，在进化过程中早期就出现了分歧。
The gene encoding the human puromycin-sensitive aminopeptidase (PSA) has been cloned and characterized. The humanPSAgene is composed of 23 exons and 22 introns and spans approximately 40 kb of chromosome 17 at the interval 17q12-21. An analysis of the 5′ end of the humanPSAtranscript reveals that the translational start site corresponds to nt 210 of the humanPSAcDNA, as suggested by RT-PCR, 5′ RACE, and computer analysis of expressed sequence tags. A comparison of the exon/exon boundaries of the humanPSAgene with those of the humanaminopeptidase N(APN) gene shows little conservation, suggesting that the two genes, which are closely related in protein sequence, diverged early during evolution.