EVIDENCE THE YEAST STE3 GENE ENCODES A RECEPTOR FOR THE PEPTIDE PHEROMONE A FACTOR - GENE SEQUENCE AND IMPLICATIONS FOR THE STRUCTURE OF THE PRESUMED RECEPTOR
EVIDENCE THE YEAST STE3 GENE ENCODES A RECEPTOR FOR THE PEPTIDE PHEROMONE A FACTOR - GENE SEQUENCE AND IMPLICATIONS FOR THE STRUCTURE OF THE PRESUMED RECEPTOR
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DOI：
10.1073/pnas.83.5.1418
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发表时间：
1986-03-01
影响因子：
11.1
通讯作者：
SPRAGUE, GF
中科院分区：
文献类型：
--
作者：
HAGEN, DC;MCCAFFREY, G;SPRAGUE, GF
a交配型的单倍体酵母细胞分泌肽信息素，一种作用于α交配型酵母细胞的因子。交配类型，以准备他们的共轭。我们表明，仅由α.细胞中，并且仅在α中转录。细胞，可能编码一种因子的细胞表面受体。这一观点基于三项调查结果。首先，野生型Ste 3产物是对信息素的反应所必需的：具有五种不同ste 3突变中的任何一种的突变体对因子无反应。第二，混合STe 3-β-由STE 3-lacZ基因融合体编码的半乳糖苷酶蛋白分级分离为酵母细胞提取物的颗粒级分，表明Ste 3是膜蛋白。最后，我们在这里报道的STE 3的DNA序列编码一种470个氨基酸残基的蛋白质，该蛋白质含有7个不同的疏水片段，其长度足以跨越脂质双层。
Haploid yeast cells of the a mating type secrete a peptide pheromone, a factor, which acts on cells of the .alpha. mating type to prepare them for conjugation. We show that the STE3 gene, which is required for mating only by .alpha. cells and is transcribed only in .alpha. cells, likely encodes a cell-surface receptor for a factor. This view is based on three findings. First, wild-type Ste3 product is required for response to the pheromone: mutants with any one of five different ste3 mutations are unresponsive to a factor. Second, a hybrid STe3-.beta.-galactosidase protein encoded by a STE3-lacZ gene fusion fractionates to the particulate fraction of yeast cell extracts, suggesting that Ste3 is a membrane protein. Finally, the DNA sequence of STE3, which we report here, encodes a protein of 470 amino acid residues that contains seven distinct hydrophobic segments of sufficient length to span a lipid bilayer.