Crystallization and preliminary X-ray studies of ferric uptake regulator from Magnetospirillum gryphiswaldense.
Crystallization and preliminary X-ray studies of ferric uptake regulator from Magnetospirillum gryphiswaldense.
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DOI：
10.1107/s1744309112024311
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Zhao Liu;Zhongzhou Chen;Wei Wu
中科院分区：
文献类型：
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作者：
Zhao Liu;Zhongzhou Chen;Wei Wu
趋磁细菌中的磁小体在磁畴研究和商业应用中有着广泛的应用。趋磁细菌的铁含量比大肠杆菌高100倍。即使在高细胞内浓度下，磁藻MSR-1仍然可以吸收铁。铁吸收调节因子（Fur）是一种全局性的铁响应调节因子，影响磁小体的形成、铁转运和氧代谢。然而，M. gryphiswaldense MSR-1毛皮不清楚。本文对M. gryphiswaldense MSR-1。这些晶体属于空间群C2。马修斯系数分析和分子排阻色谱分析表明，不对称单元中可能含有一个二聚体。将衍射数据优化为apo Fur的1.58 nm分辨率和SeMet Fur的1.9 nm分辨率。
Magnetosomes in magnetotactic bacteria have been widely used in studies of magnetic domains and in commercial applications. The iron content of magnetotactic bacteria is ∼100 times higher than that of Escherichia coli. Magnetospirillum gryphiswaldense MSR-1 can still take up iron even at high intracellular concentrations. Ferric uptake regulator (Fur) is a global iron-responsive regulator that affects magnetosome formation, iron transport and oxygen metabolism. However, the mechanism of iron uptake and homeostasis by M. gryphiswaldense MSR-1 Fur is not clear. Here, the expression, purification and crystallization of apo and SeMet Fur from M. gryphiswaldense MSR-1 are reported. The crystals belonged to space group C2. Matthews coefficient analysis and size-exclusion chromatography showed that the asymmetric unit probably contains one dimer of Fur. Diffraction data were optimized to 1.58 Å resolution for apo Fur and to 1.9 Å resolution for SeMet Fur.