Quantitative Analyses of F?rster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo‐FRET) In DNA Scaffolds.
Quantitative Analyses of F?rster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo‐FRET) In DNA Scaffolds.
复制标题
DNA 支架中相同芘发色团 (Homo-FRET) 之间 F?rster 共振能量转移的定量分析。
DOI：
10.1002/cptc.202000199
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Asanuma Hiroyuki
中科院分区：
文献类型：
--
作者：
Kashida Hiromu;Kawai Hayato;Azuma Hidenori;Araki Yasuyuki;Wada Takehiko;Asanuma Hiroyuki
相同发色团(HOMO-FRET)之间的Förster共振能量转移一直很难分析，因为发射强度和寿命都不会随着HOMO-FRET的出现而改变。在这里，我们使用了DNA支架来分析芘部分之间的同源FRET。对DNA支架进行了修饰，加入了两种芘和一种猝灭剂--蒽醌。通过监测荧光猝灭和荧光寿命的缩短来检测Homo-FRET。同源FRET的效率可以通过排除异源FRET的影响来计算。实验确定的效率与Förster理论符合得很好。这些结果将为新型分子探测器和捕光天线的设计提供依据。
Förster resonance energy transfer between identical chromophores (homo‐FRET) has been difficult to analyze since neither emission intensity nor lifetime changes with the occurrence of homo‐FRET. Herein we used a DNA scaffold to analyze homo‐FRET between pyrene moieties. The DNA scaffold was modified with two pyrenes and a quencher, anthraquinone. Homo‐FRET was detected by monitoring quenching of pyrene emission and the decrease in the fluorescence lifetime of pyrene. Homo‐FRET efficiencies could be calculated by excluding effects of hetero‐FRET. The experimentally determined efficiencies showed an excellent agreement with Förster theory. These results will inform design of novel molecular probes and light‐harvesting antennae.