ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling.
ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling.
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ZNF545 通过抑制 NF-kB 信号传导抑制人肝细胞癌生长
DOI：
10.18632/genesandcancer.137
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Guo M
中科院分区：
文献类型：
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作者：
Yang W;Yang S;Zhang M;Gao D;He T;Guo M
肝细胞癌（HCC）是世界范围内最常见的癌症之一，也是癌症相关死亡的第二大原因。ZNF 545位于染色体19q13.13，是星形细胞瘤中常见的杂合性丢失位点。ZNF 545甲基化在少数几种肿瘤中存在。然而ZNF 545在人HCC中的功能尚不清楚。本研究旨在探讨ZNF 545在人肝癌中的作用及其机制。恢复ZNF 545的表达可抑制SNU 449和Huh 7细胞的增殖、迁移和侵袭，诱导细胞G1/S期阻滞和凋亡。进一步研究表明ZNF 545通过抑制NF-κ B信号通路抑制肝癌细胞生长。在ZNF 545高表达的HXBF 344细胞中采用siRNA敲低技术进一步验证了上述结果。在体内，ZNF 545抑制SNU 449细胞异种移植小鼠的肿瘤生长。总之，ZNF 545通过抑制NF-κ B信号转导抑制人HCC生长。
Hepatocellular carcinoma (HCC) is one of the most common cancers and the second leading cause of cancer related death worldwide. ZNF545 is located in the chromosome 19q13.13, which is frequent loss of heterozygosity in human astrocytoma. Methylation of ZNF545 was found frequently in a few kinds of cancers. While the function of ZNF545 in human HCC remains unclear. The purpose of this study is to explore the function and mechanism of ZNF545 in human HCC. Restoration of ZNF545 expression suppressed cell proliferation, migration and invasion, induced G1/S arrest and apoptosis in SNU449 and Huh7 cells. Further study suggested that ZNF545 suppressed HCC cell growth by inhibiting NF-kB signaling. These results were further validated by siRNA knocking down technique in ZNF545 highly expressed HXBF344 cells. In vivo, ZNF545 suppressed tumor growth in SNU449 cell xenograft mice. In conclusion, ZNF545 suppresses human HCC growth by inhibiting NF-kB signaling.