Platelet interactions with titanium: modulation of platelet activity by surface topography
Platelet interactions with titanium: modulation of platelet activity by surface topography
复制标题
DOI：
10.1016/s0142-9612(01)00009-6
复制
发表时间：
2001-10-01
期刊：
影响因子：
14
通讯作者：
Davies, JE
中科院分区：
文献类型：
--
作者：
Park, JY;Gemmelll, CH;Davies, JE
骨内植入物最初与血液接触。因此，血液和植入的骨内植入物之间的相互作用的性质可能会影响植入物周围愈合室中随后的骨愈合事件。我们进行了研究，以解决以下问题：种植体表面微观结构是否调节血小板活性？我们使用了具有四种不同表面光洁度的商业纯钛（cpTi）盘：双重酸蚀刻（DAE）、320粒度（320 G）研磨、机加工和p1200抛光cpTi。通过扫描电子显微镜（SEM）和光学轮廓仪对表面进行了表征。DAE和320 G表面呈现出比机加工或抛光表面更复杂的微观织构。通过定量血小板粘附、血小板衍生微粒（MP）形成和P-选择素表达作为表面类型的函数来测量血小板活性。使用乳酸脱氢酶（LDH）测定法测量的血小板粘附在DAE和320 G表面上比机加工和抛光表面上增加（p < 0.05）。MP形成和P-选择素的表达，流式细胞仪测定，也表明DAE和320 G表面上的血小板活化增加。由于血小板活化的增加可能导致骨愈合过程中成骨反应的上调，因此这些结果可以解释DAE cpTi表面与机加工cpTi表面相比已知发生的骨传导性增强。(C)2001爱思唯尔科技有限公司版权所有。
Endosseous implants initially come into contact with blood. Thus, the nature of the interactions between blood and implanted endosseous implants may influence subsequent bone healing events in the peri-implant healing compartment. We conducted studies to address the following question: Does implant surface microtexture modulate platelet activity? We used commercially pure Ti (cpTi) disks with four different surface finishes: dual acid-etched (DAE), 320 grit (320G) abraded, machined, and p1200 polished cpTi. Surfaces were characterized by scanning electron microscopy (SEM) and optical profilometry. The DAE and 320G surfaces presented more complex microtextures than the machined or polished surfaces. Platelet activities were measured by quantifying platelet adherence, platelet-derived microparticle (MP) formation, and P-selectin expression as function of surface type. Platelet adhesion, measured using a lactate dehydrogenase (LDH) assay, was increased on DAE and 320G surfaces compared to machined and polished surfaces (p < 0.05). MP formation and P-selectin expression, assayed by flow cytometry, also showed increased activation of platelets on DAE and 320G surfaces. Because increased activation of platelets may lead to up-regulation of osteogenic responses during bone healing, these results may explain the enhanced osteoconductivity known to occur with DAE cpTi surfaces in comparison with machined cpTi surfaces. (C) 2001 Elsevier Science Ltd. All rights reserved.