Guanine nucleotide regulation of [125I]beta-endorphin binding to NG108-15 and SK-N-SH cell membranes: specific cation requirements.
Guanine nucleotide regulation of [125I]beta-endorphin binding to NG108-15 and SK-N-SH cell membranes: specific cation requirements.
复制标题
[125I]β-内啡肽与 NG108-15 和 SK-N-SH 细胞膜结合的鸟嘌呤核苷酸调节：特定阳离子要求。
DOI：
10.1016/0006-8993(89)90996-7
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Bidlack,JM
中科院分区：
文献类型：
--
作者：
Selley,DE;Bidlack,JM
Receptor of [125Iβ-endorphin binding by nucleotides was investigated in membrane preparations from two opioid receptor-containing cell lines: NG108-15, which contains only δ opioid receptors, and SK-N-SH, which contains predominantly μ opioid receptors. In contrast to the binding of the σ-selective agonist [3H][d-penicillamine2,d-penicillamine5]enkephalin to to NG108-15 cell membranes, and of the μ-selective agonist [3H][d-Ala2, MePhe4, Gly-ol5]enkephalin to SK-N-SH cell membranes. [125Iβh-endorphin binding to NG108-15 and SK-N-SH cell membranes was not altered by guanosine triphosphate (GTP) or guanylyl-5'-imidodiphosphate (Gpp(NH)p) in the absence of cations. However, in the presence of NaCl, [125I]βh-endorphin binding to both cell lines was inhibited by GTP and Gpp(NH)p in a concentration-dependent manner. In SK-H-SH cell membranes, the ability of sodium to promote regulation of [125I]βh-endorphin binding by GTP was mimicked by the monovalent cations lithium and potassium, but not by the divalent cations magnesium, calcium, or mangenese. In NG108-15 cells membranes, only sodium was effective in promoting inhibition of [125I]βh-endorphin binding by GTP or Gpp(NH)p in the presence of sodium was also observed with guanosine diphosphate, but not guanosine monophosphate or any of the non-guanine nucleotides tested. These results indicate that the presence of monovalent cations is required for regulation of [125I]βh-endorphin binding by guanine nucleotides, and that the specificity of this cation requirement differs between the μ and δ receptor-containing cell lines.
