AMINO-ACID SUBSTITUTION MATRICES FROM PROTEIN BLOCKS
AMINO-ACID SUBSTITUTION MATRICES FROM PROTEIN BLOCKS
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DOI：
10.1073/pnas.89.22.10915
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发表时间：
1992-11-15
影响因子：
11.1
通讯作者：
HENIKOFF, JG
中科院分区：
文献类型：
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作者：
HENIKOFF, S;HENIKOFF, JG
蛋白质序列比对方法通常通过使用一个替换矩阵来衡量相似性，该矩阵包含一种氨基酸与另一种氨基酸所有可能替换的得分。最广泛使用的矩阵是基于戴霍夫（Dayhoff）进化速率模型。我们采用一种不同的方法，从大约2000个比对的序列片段模块中推导出替换矩阵，这些模块描述了500多组相关蛋白质的特征。这使得比对以及使用来自每组的查询进行搜索都有了显著的改进。
Methods for alignment of protein sequences typically measure similarity by using a substitution matrix with scores for all possible exchanges of one amino acid with another. The most widely used matrices are based on the Dayhoff model of evolutionary rates. Using a different approach, we have derived substitution matrices from about 2000 blocks of aligned sequence segments characterizing more than 500 groups of related proteins. This led to marked improvements in alignments and in searches using queries from each of the groups.