Stress-free automatic sleep deprivation using air puffs.
Stress-free automatic sleep deprivation using air puffs.
复制标题
使用空气吹气的无压力自动睡眠剥夺。
DOI：
10.1016/j.jneumeth.2015.05.010
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发表时间：
2015-08-15
影响因子：
3
通讯作者：
Poe GR
中科院分区：
文献类型：
--
作者：
Gross BA;Vanderheyden WM;Urpa LM;Davis DE;Fitzpatrick CJ;Prabhu K;Poe GR
通过温和的处理剥夺睡眠是耗时和人员密集型的。我们在这里提出了一个自动睡眠剥夺系统通过空气泡芙。植入EMG和EEG电极用于评估6只雄性Sprague-Dawley大鼠的睡眠/清醒状态。在基线的12小时光照周期期间，每4小时从植入的静脉内导管收集血液样品，通过空气抽吸进行8小时睡眠剥夺，以及通过温和处理进行8小时睡眠剥夺。自动化系统能够像我们的离线版本一样准确地对睡眠和清醒状态进行评分（睡眠约90%），并且具有足够的速度在可接受的时间内（1.76秒）触发反馈响应。手动状态评分确认了基线日的正常睡眠和两个操作日的睡眠剥夺（非REM减少68%，REM减少63%，清醒时增加74%）。在基线睡眠和通过空气抽吸剥夺睡眠之间的任何时间点，ACTH和皮质酮（指示HPA轴活动的应激激素）水平均无显著差异。在8小时的时间内，空气吹和温和处理睡眠剥夺之间的ACTH或皮质酮浓度没有显着差异。我们的系统准确地检测睡眠，并提供空气喷急性剥夺大鼠的睡眠与足够的时间分辨率在关键的4-5小时学习后睡眠依赖的记忆巩固期。该系统是无压力的，是现有睡眠剥夺技术的可行替代方案。
Sleep deprivation via gentle handling is time-consuming and personnel-intensive. We present here an automated sleep deprivation system via air puffs. Implanted EMG and EEG electrodes were used to assess sleep/waking states in six male Sprague-Dawley rats. Blood samples were collected from an implanted intravenous catheter every 4 hours during the 12-hour light cycle on baseline, 8 hours of sleep deprivation via air puffs, and 8 hours of sleep deprivation by gentle handling days. The automated system was capable of scoring sleep and waking states as accurately as our offline version (~90% for sleep) and with sufficient speed to trigger a feedback response within an acceptable amount of time (1.76 s). Manual state scoring confirmed normal sleep on the baseline day and sleep deprivation on the two manipulation days (68% decrease in non-REM, 63% decrease in REM, and 74% increase in waking). No significant differences in levels of ACTH and corticosterone (stress hormones indicative of HPA axis activity) were found at any time point between baseline sleep and sleep deprivation via air puffs. There were no significant differences in ACTH or corticosterone concentrations between sleep deprivation by air puffs and gentle handling over the 8-hour period. Our system accurately detects sleep and delivers air puffs to acutely deprive rats of sleep with sufficient temporal resolution during the critical 4-5 h post learning sleep-dependent memory consolidation period. The system is stress-free and a viable alternative to existing sleep deprivation techniques.