Time dependence of singlet oxygen luminescence provides an indication of oxygen concentration during oxygen consumption
Time dependence of singlet oxygen luminescence provides an indication of oxygen concentration during oxygen consumption
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DOI：
10.1117/1.2821153
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发表时间：
2007-11-01
影响因子：
3.5
通讯作者：
Baeumler, Wolfgang
中科院分区：
文献类型：
--
作者：
Baier, Juergen;Maisch, Tim;Baeumler, Wolfgang
单线态氧在肿瘤细胞或细菌的光动力灭活中起主要作用。它的疗效主要取决于氧浓度[O-2]，如果氧化反应导致氧气消耗，氧浓度[O-2]会降低。当利用单线态氧在1270 nm处的发光直接检测单线态氧时，发光信号的进程受到[O-2]的严重影响。因此，监测光氧化过程中的氧气消耗应该是可行的。在白蛋白水溶液(H2O或D2O)中激发光敏剂(TMPyP)产生单线态氧。层析表明，大多数TMPyP分子是无结合的，因此单线态氧分子可以在溶液中扩散。氧浓度传感器装置显示，在照射期间，溶液中的[O-2](氧气消耗)迅速减少。白蛋白水溶液中氧气的耗尽程度取决于辐射照射和溶剂。在探测单线态氧的发光信号时，发光信号的形状随着辐照时间的延长而发生明显的变化。因此，可以通过评估单线态氧的发光过程来监测光动力作用期间的局部耗氧量。(C)2007年，光学仪器工程师学会。
Singlet oxygen plays a major role in photodynamic inactivation of tumor cells or bacteria. Its efficacy depends critically on the oxygen concentration [O-2], which can decrease in case oxygen is consumed caused by oxidative reactions. When detecting singlet oxygen directly by its luminescence at 1270 nm, the course of the luminescence signal is critically affected by [O-2]. Thus, it should be feasible to monitor oxygen consumption during photo-oxidative processes. Singlet oxygen was generated by exciting a photosensitizer (TMPyP) in aqueous solution (H2O or D2O) of albumin. Chromatography shows that most of the TMPyP molecules are unbound, and therefore singlet oxygen molecules can diffuse in the solution. A sensor device for oxygen concentration revealed a rapid decrease of [O-2] (oxygen depletion) in the solution during irradiation. The extent of oxygen depletion in aqueous albumin solution depends on the radiant exposure and the solvent. When detecting the luminescence signal of singlet oxygen, the shape of the luminescence signal significantly changed with irradiation time. Thus, local oxygen consumption could be monitored during photodynamic action by evaluating the course of singlet oxygen luminescence. (C) 2007 Society of Photo-Optical Instrumentation Engineers.