Functional Expression of the Ectodomain of Plant Receptor Kinases in Plant Suspension Culture
Functional Expression of the Ectodomain of Plant Receptor Kinases in Plant Suspension Culture
复制标题
植物悬浮培养中植物受体激酶胞外域的功能表达
DOI：
10.1007/978-1-0716-3147-8_7
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发表时间：
2023
影响因子：
--
通讯作者：
Shinohara Hidefumi
中科院分区：
文献类型：
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作者：
Shinohara Hidefumi
细胞外信号通常由膜定位的受体感知，这些受体调节细胞间信号以激活各种途径。在植物中，单跨膜受体激酶作为细胞外信号的受体。植物内源分泌肽类激素是一种新型的信号分子，通过配体-受体对的形成发挥作用。近年来，基于结构生物学方法的植物肽受体相互作用的研究取得了很大进展，但利用杆状病毒表达系统在昆虫细胞中表达的重组受体分子的解离常数相对较低。本文介绍了一种利用烟草BY-2细胞建立稳定的、功能性的植物受体激酶均相表达系统的方法，同时保持了传统的配体结合活性。这一策略将有助于提高我们对植物内源肽配体-受体相互作用的理解。
Extracellular signals are usually perceived by membrane-localized receptors that transduce intercellular signals to activate various pathways. In plants, single transmembrane receptor kinases act as receptors for extracellular signals. Endogenous secreted peptide hormones have been recognized as novel signaling molecules, functioning through the formation of ligand-receptor pairs in plants. Recently, research on plant peptide hormone-receptor interactions based on the structural biology approach has greatly improved; however, the dissociation constant of recombinant receptor molecules expressed in insect cells using the baculovirus expression system is relatively low. We introduce here a method for creating a stable and functional homogeneous expression system for plant receptor kinases using tobacco BY-2 cells while maintaining conventional ligand-binding activity. This strategy will help improve our understanding of plant endogenous peptide ligand-receptor interactions.