Effect of Chloride on Ferrous Iron Oxidation by a Leptospirillum ferriphilum-Dominated Chemostat Culture
Effect of Chloride on Ferrous Iron Oxidation by a Leptospirillum ferriphilum-Dominated Chemostat Culture
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DOI：
10.1002/bit.22709
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发表时间：
2010-06-15
影响因子：
3.8
通讯作者：
Sandstrom, Ake
中科院分区：
文献类型：
--
作者：
Gahan, Chandra Sekhar;Sundkvist, Jan-Eric;Sandstrom, Ake
生物采矿是利用微生物催化从硫化矿石中提取金属。然而，在一些生物矿化环境中，可用的水具有较高的氯化物浓度，因此，氯化物对氧化亚铁微生物的毒性已被研究。12gL（-1）氯化物完全抑制了嗜铁钩端螺旋体对Fe2+的批量生物氧化。此外，还研究了氯化物对Fe2+受限趋化器氧化动力学的影响。化学调节模型的结果表明，氯化物毒性归因于对Fe2+氧化系统、pH稳态和质子动力降低的影响。模型显示，随着氯浓度的增加，最大比生长率（mu(max)）降低，底物常数（K-s）增加，表明对Fe2+氧化系统有影响。该模型表明，当培养基中添加2-3 g L-1氯化物时，维持活性降低，同时真实产量（Y-true）急剧下降。该模型有助于理解氯化物对Fe2+生物氧化动力学的影响。Biotechnol。Bioeng。2010;106: 422 - 431。(C) 2010 Wiley期刊公司
Biomining is the use of microorganisms to catalyze metal extraction from sulfide ores. However, the available water in some biomining environments has high chloride concentrations and therefore, chloride toxicity to ferrous oxidizing microorganisms has been investigated. Batch biooxidation of Fe2+ by a Leptospirillum ferriphilum dominated culture was completely inhibited by 12gL(-1) chloride. In addition, the effects of chloride on oxidation kinetics in a Fe2+ limited chemostat were studied. Results from the chemostat modeling suggest that the chloride toxicity was attributed to affects on the Fe2+ oxidation system, pH homeostasis, and lowering of the proton motive force. Modeling showed a decrease in the maximum specific growth rate (mu(max)) and an increase in the substrate constant (K-s) with increasing chloride concentrations, indicating an effect on the Fe2+ oxidation system. The model proposes a lowered maintenance activity when the media was fed with 2-3 g L-1 chloride with a concomitant drastic decrease in the true yield (Y-true). This model helps to understand the influence of chloride on Fe2+ biooxidation kinetics. Biotechnol. Bioeng. 2010;106: 422-431. (C) 2010 Wiley Periodicals, Inc.