A Comparative Evaluation of the Antiproliferative Activity against HepG2 Liver Carcinoma Cells of Plant-Derived Silver Nanoparticles from Basil Extracts with Contrasting Anthocyanin Contents
A Comparative Evaluation of the Antiproliferative Activity against HepG2 Liver Carcinoma Cells of Plant-Derived Silver Nanoparticles from Basil Extracts with Contrasting Anthocyanin Contents
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DOI：
10.3390/biom9080320
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发表时间：
2019-08-01
期刊：
影响因子：
5.5
通讯作者：
Hano, Christophe
中科院分区：
文献类型：
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作者：
Abbasi, Bilal Haider;Nazir, Munazza;Hano, Christophe
纳米技术是一个成熟的和革命性的领域，具有不同的治疗特性。已经采用使用不同还原剂的几种方法来合成银纳米颗粒（AgNP）。化学介导的合成方法是有毒的，并导致对生物系统的非预期影响。在本文中，我们使用紫罗勒的愈伤组织提取物（BC-AgNP）和源自相同植物的花青素苷提取物（即，紫罗勒）（AE-AgNPs），并系统地研究了它们对HepG 2肝癌细胞的抗增殖潜力。采用紫外-可见光谱（UV-Vis）、X射线衍射（XRD）、傅立叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和能量色散X射线（EDX）等技术对植物合成的AgNPs进行表征。在形态学上，两种类型的NP均为球形。通过XRD和SEM分析显示的BC-AgNP和AE-AgNP的平均尺寸分别计算为50.97 +/- 0.10 nm和42.73 +/- 1.24 nm。FT-IR光谱分析表明存在可能的植物化学物质所需的银离子的上限和减少。在此，固相萃取（SPE）结合HPLC分析，我们首次报告了花青素介导的银纳米颗粒的合成，并确认花青素的成功盖帽。与BC-AgNPs相比，小尺寸AE-AgNPs显示出对人肝细胞癌（HepG 2）细胞系的显著细胞毒性作用。因此，结果表明，紫罗勒中存在的黄酮类化合物的普遍组是花青素，并且AE-AgNPs可以在未来的治疗策略中用作潜在的抗癌剂。
Nanotechnology is a well-established and revolutionized field with diverse therapeutic properties. Several methods have been employed using different reducing agents to synthesize silver nanoparticles (AgNPs). Chemical mediated synthetic methods are toxic and resulted in non-desired effects on biological systems. Herein, we, synthesized silver nanoparticles using callus extract of purple basil (BC-AgNPs) and anthocyanin extract deriving from the same plant (i.e., purple basil) (AE-AgNPs), and systematically investigated their antiproliferative potential against HepG2 Liver Carcinoma Cells. The phyto-fabricated AgNPs were characterized by different techniques like UV-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM) and Energy dispersive X-rays (EDX). Morphologically, both types of NPs were found spherical. The average size of BC-AgNPs and AE-AgNPs as revealed through XRD and SEM analyses were calculated as 50.97 +/- 0.10 nm and 42.73 +/- 1.24 nm, respectively. FT-IR spectral analysis demonstrates the existence of possible phytochemicals required for the capping and reduction of Ag ions. Herein, following solid phase extraction (SPE) coupled to HPLC analysis, we report for the first-time the anthocyanin mediated synthesis of AgNPs and conforming the successful capping of anthocyanin. Small sized AE-AgNPs showed significant cytotoxic effect against human hepatocellular carcinoma (HepG2) cell line as compared to BC-AgNPs. Therefore, the results revealed that the prevalent group of flavonoids present in purple basil is the anthocyanins and AE-AgNPs could be employed as potential anticancer agents in future treatments strategies.