A silica nanoparticle-based dual-responsive ratiometric probe for visualizing hypochlorite and temperature with distinct fluorescence signals
A silica nanoparticle-based dual-responsive ratiometric probe for visualizing hypochlorite and temperature with distinct fluorescence signals
复制标题
基于二氧化硅纳米粒子的双响应比例探针，用于通过不同的荧光信号可视化次氯酸盐和温度
DOI：
10.1016/j.snb.2017.05.072
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Jianhui Jiang
中科院分区：
文献类型：
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作者：
Jian Chen;Chonghua Zhang;Kun Lv;Hong Wang;Peisheng Zhang;Pinggui Yi;Jianhui Jiang
次氯酸盐（ClO−）和温度在广泛的生理过程中起着至关重要的作用，它们也涉及多种疾病，包括癌症、组织炎症等。因此，探索一种实时检测ClO−和温度的新方法具有重要的意义。在这项研究中，我们开发了一种基于二氧化硅纳米颗粒的双响应比例荧光传感器（DRFS），其相关的双发射可以独立灵敏地响应ClO -和温度。DRFS对ClO−的检测限可低至26 nM。进一步的研究表明，在存在其他可能干扰的情况下，DRFS具有良好的抗干扰特性，并已成功应用于人血清中ClO−的检测以及HeLa细胞和巨噬细胞中外源性/内源性ClO−的荧光显微成像识别。此外，DRFS还可以用作比例温度传感器，其荧光强度比（I576/I445）在20 ~ 60℃范围内呈线性温度响应，变化率可达5倍。基于以上研究，DRFS可作为多功能荧光传感器应用于各种生理和环境系统中。
Hypochlorite (ClO−) and temperature play crucial roles in a wide range of physiological processes, and they are also implicated in various diseases, including cancer, inflammation of tissues and so on. Therefore, it is of great importance to explore a novel method to detect ClO−and temperature instantly. In this study, we developed a silica nanoparticle-based dual-responsive ratiometric fluorescent sensor (DRFS), whose correlative dual emissions can response to ClO−and temperature independently and sensitively. The detection limit of DRFS can reach to as low as 26 nM for the detection of ClO−. And further research demonstrates that DRFS possesses excellent anti-interference feature when other possible interferents exist, and has been successfully applied in ClO−detection in human serum and recognition of exogenous/endogenous ClO−in HeLa cells and macrophages by fluorescence microscopic imaging. Moreover, DRFS can also be used as a ratiometric temperature sensor, and the fluorescence intensity ratio (I576/I445) exhibits a linear temperature response in the range from 20 to 60 °C with a change ratio as large as a factor of 5. Based on the above research, the DRFS can be used as versatile fluorescence sensor in various physiological and environmental systems.